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| Status |
Public on Feb 16, 2024 |
| Title |
Cardiomyocyte-specific adenylyl cyclase type-8 overexpression induces cell-autonomous activation of RelA and non-cell-autonomous myocardial and systemic inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using a model of cardiac AC8 overexpression that lead to accelerated cardiac aging, fibrosis and immune system overactivation, this study used scRNA-seq to investigate the signaling pathways dysregulated in cardiac cells to have a better understanding of the biological processes invoved in cardiac aging.
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| Overall design |
A single cell suspension was made from fresh cardiac tissue obtained from AC8 overexpressing mice and littermate controls, An equal number of FACS-sorted CD45+CD31- (leukocytes), CD45-CD31- (resident mesenchymal cells), and CD45-CD31+ (endothelial cells) cells were mixed and processed to generate single-cell cDNA libraries using the 10X Genomics platform Cells were sequenced at a target read depth of 75,000 reads/cell using a NovaSeq 6000 sequencer (Illumina). Single-cell counts were generated from unaligned reads by sequence alignment and de-multiplexing using Cell Ranger from 10x Genomics.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/38499959/
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| Contributor(s) |
Kumar V, Bermea KC, Kumar D, Singh A, Verma A, Kaileh M, Sen R, Lakatta EG, Luigi A |
| Citation(s) |
38499959 |
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| Submission date |
Feb 16, 2024 |
| Last update date |
May 17, 2024 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
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| Department |
Laboratory of Genetics and Genomics
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| Lab |
Computational Biology & Genomics Core
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| Street address |
251 Bayview Blvd
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| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
| GSM8082522 |
C57/B6 mice, pooled, cardiac cell suspension |
| GSM8082523 |
AC8 Overexpressing C57/B6 mice, pooled, cardiac cell suspension |
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| Relations |
| BioProject |
PRJNA1077211 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE255916_RAW.tar |
23.2 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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