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| Status |
Public on Aug 07, 2024 |
| Title |
The diadenosine tetraphosphate hydrolase ApaH is crucial for Pseudomonas aeruginosa pathogenicity |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The role of the intracellular signaling molecule diadenosine tetraphosphate (Ap4A) has not so far been investigated in Pseudomonas aeruginosa. To fill this gap, we performed RNA sequencing (RNA-seq) analysis to compare the transcriptome of the reference strain P. aeruginosa PAO1 and an isogenic deletion mutant (ΔapaH) which is deficient in the Ap4A hydrolysing enzyme ApaH and accumulates high intracellular levels of Ap4A.
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| |
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| Overall design |
2 different groups with 3 biological replicates each. Group A: wild type strain. Group B: apaH mutant strain.
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| |
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| Contributor(s) |
Cervoni M, Sposato D, Visca P, Leoni L, Rampioni G, Imperi F |
| Citation(s) |
39159286 |
| |
| Submission date |
Feb 15, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Francesco Imperi |
| E-mail(s) |
francesco.imperi@uniroma3.it
|
| Phone |
+39 0657336347
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| Organization name |
Roma Tre University
|
| Department |
Department of Scienze
|
| Lab |
Laboratory of Microbiology
|
| Street address |
Viale Marconi, 446
|
| City |
Rome |
| State/province |
Rome |
| ZIP/Postal code |
00146 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL26913 |
Illumina NovaSeq 6000 (Pseudomonas aeruginosa) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1076844 |
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