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Series GSE25549 Query DataSets for GSE25549
Status Public on Nov 23, 2011
Title Polycomb Protein Ezh1 Promotes RNA Polymerase II Elongation
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Polycomb group (PcG) proteins initiate the formation of repressed chromatin domains and regulate developmental gene expression. A mammalian PcG protein, Enhancer of Zeste homolog 2 (Ezh2), triggers transcriptional repression by catalyzing the addition of methyl groups onto lysine-27 of histone H3 (H3K27me2/3)1. This action facilitates the binding of other PcG proteins to histone H3 and compaction of chromatin. Interestingly, there exists a paralog of Ezh2, termed Ezh1, whose primary function remains unclear. Here, we provide evidence for genome-wide association of Ezh1 with active epigenetic marks, RNA polymerase II (PolII) and mRNA production. Ezh1 depletion reduced global PolII occupancy within gene bodies and resulted in delayed transcriptional activation during differentiation of skeletal muscle cells. Conversely, ectopic expression of wild-type Ezh1 led to premature gene activation and rescued PolII-elongation defects in Ezh1-depleted cells. Collectively, these findings reveal an unanticipated role of a PcG protein in promoting mRNA transcription.
Overall design Examination of 3 different histone modifications, 3 modified forms of RNA polymerase II, Ezh1, Ezh2 and mRNA levels in a skeletal muscle cells at various developmental stages.
Contributor(s) Mousavi K, Zare H, Wang AH, Sartorelli V
Citation(s) 22196887
Submission date Nov 22, 2010
Last update date May 15, 2019
Contact name Kambiz Mousavi
Phone 301-435-5648
Fax 301-480-9699
Organization name National Institutes of Health
Department National Institute of Arthritis and Musculoskeletal and Skin Diseases
Lab Laboratory of Muscle Stem Cells and Gene Regulation
Street address 50 South Drive, Room 1347
City Bethesda
State/province MD
ZIP/Postal code 20892-8024
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (30)
GSM628005 MB_H3K4me3_ChIPSeq
GSM628006 MT_H3K4me3_ChIPSeq
GSM628007 MB_H3K27me3_ChIPSeq
BioProject PRJNA134029
SRA SRP004636

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Supplementary file Size Download File type/resource
GSE25549_RAW.tar 6.4 Gb (http)(custom) TAR (of BED, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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