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Series GSE25536 Query DataSets for GSE25536
Status Public on Oct 16, 2016
Title Expression profiling in primary embryonic fibroblasts deficient for the lats2 locus
Organism Mus musculus
Experiment type Expression profiling by array
Summary We previously reported LATS2 null mice (Yabuta et al., 2007). LATS2 known as a tumor suppressor protein. However, the LATS2 mediated transcriptional regulation remains largely unknown. To investigate potential signaling pathways, we performed the expression profiling by using primary MEFs derived from wild type and LATS2 null mice.
Overall design Total RNAs were extracted from MEFs derived from LATS2+/+ and LATS2-/- C57BL/6J mice using miRNeasy extraction kit (Qiagen). A Dye-swapped experiment was performed by hybridizing complimentary RNA (cRNA) labeled with either Cyanine (Cy) -3 or Cy-5 (Perkin-Elmer) onto Whole Mouse Genome Oligo Microarray (G4122F; Agilent Technologies).
Contributor(s) Okuzaki D, Nojima H
Citation(s) 27434182
Submission date Nov 22, 2010
Last update date Jan 12, 2017
Contact name Daisuke Okuzaki
Phone +81-6-6879-4935
Organization name Osaka univ.
Department Immunology Frontier Research Center
Lab Human Immunology (Single Cell Genomics)
Street address Yamadaoka 3-1
City Suita
State/province Osaka
ZIP/Postal code 565-0871
Country Japan
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (2)
GSM627707 LATS2+/+vsLATS2-/-
GSM627708 LATS2-/-vsLATS2+/+
BioProject PRJNA134007

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25536_RAW.tar 28.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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