|
| Status |
Public on May 17, 2011 |
| Title |
Single tip transcriptomics of neighboring hyphae of Aspergillus niger |
| Platform organism |
Aspergillus niger CBS 513.88 |
| Sample organism |
Aspergillus niger |
| Experiment type |
Expression profiling by array
|
| Summary |
Mycelia of filamentous fungi explore new substrates by means of hyphae that extend from the periphery of the colony. Previously, it has been shown by immuno-labelling, reporter studies and in situ hybridization that these exploring hyphae are heterogenic with respect to protein secretion and transcription.
We performed single hyphal tip RNA profiling using microarrays to assess the differences in RNA accumulation in neighboring exploring hyphae
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| |
|
| Overall design |
Single tips from 5 neighboring exploring hyphae from a single colony were selected for RNA extraction, amplification and hybridization on Affymetrix microarrays
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| |
|
| Contributor(s) |
de Bekker C, Bruning O, Breit TM, Wösten HA |
| Citation(s) |
21816052 |
| |
| Submission date |
Nov 19, 2010 |
| Last update date |
Oct 31, 2019 |
| Contact name |
Oskar Bruning |
| Organization name |
University of Amsterdam
|
| Department |
SILS
|
| Lab |
MicroArray Department
|
| Street address |
Science Park 904
|
| City |
Amsterdam |
| ZIP/Postal code |
1098 XH |
| Country |
Netherlands |
| |
|
| Platforms (1) |
| GPL6758 |
[dsmM_ANIGERa_anColl] DSM Aspergillus niger CBS513.88 14k v1 |
|
| Samples (5)
|
|
| Relations |
| BioProject |
PRJNA134633 |
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