|
| Status |
Public on Mar 01, 2024 |
| Title |
Experimental tests strongly challenge the evidence of a healthy human blood microbiome |
| Organisms |
Escherichia coli; Mus musculus; human blood metagenome |
| Experiment type |
Other
|
| Summary |
The study critically evaluate the results of 16S targeted amplicon sequencing performed on the total DNA collected from healthy donors’ blood samples in the light of specific negative controls.
|
| |
|
| Overall design |
Total DNA was extracted from the blood of healthy subjects and from negative controls (whose DNA concentration has been inflated through known spyke DNA), amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform.
|
| |
|
| Contributor(s) |
Ramazzotti M, Amedei A, Di Gloria L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 01, 2024 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Matteo Ramazzotti |
| E-mail(s) |
matteo.ramazzotti@unifi.it
|
| Organization name |
University of Florence
|
| Department |
Experimental and Clinical Biomedical Sciences
|
| Street address |
viale Morgagni 50
|
| City |
Firenze |
| ZIP/Postal code |
50134 |
| Country |
Italy |
| |
|
| Platforms (3) |
| GPL16085 |
Illumina MiSeq (Escherichia coli) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL34159 |
Illumina MiSeq (human blood metagenome) |
|
| Samples (15)
|
| GSM8058751 |
Reads obtained from sequencing of sample Blood1 |
| GSM8058752 |
Reads obtained from sequencing of sample Blood3 |
| GSM8058753 |
Reads obtained from sequencing of sample Blood5 |
| GSM8058754 |
Reads obtained from sequencing of sample Blood2 |
| GSM8058755 |
Reads obtained from sequencing of sample Blood4 |
| GSM8058756 |
Reads obtained from sequencing of sample Blood6 |
| GSM8058757 |
Reads obtained from sequencing of sample Blood7 |
| GSM8058758 |
Reads obtained from sequencing of sample Blood9 |
| GSM8058759 |
Reads obtained from sequencing of sample Blood10 |
| GSM8058760 |
Reads obtained from sequencing of sample E_Coli3 |
| GSM8058761 |
Reads obtained from sequencing of sample Blood8 |
| GSM8058762 |
Reads obtained from sequencing of sample Blood11 |
| GSM8058763 |
Reads obtained from sequencing of sample E_Coli4 |
| GSM8058764 |
Reads obtained from sequencing of sample Mice_DNA1 |
| GSM8058765 |
Reads obtained from sequencing of sample Mice_DNA2 |
|
| Relations |
| BioProject |
PRJNA1072063 |
Less...
More...