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Series GSE254843 Query DataSets for GSE254843
Status Public on Mar 01, 2024
Title Experimental tests strongly challenge the evidence of a healthy human blood microbiome
Organisms Escherichia coli; Mus musculus; human blood metagenome
Experiment type Other
Summary The study critically evaluate the results of 16S targeted amplicon sequencing performed on the total DNA collected from healthy donors’ blood samples in the light of specific negative controls.
Overall design Total DNA was extracted from the blood of healthy subjects and from negative controls (whose DNA concentration has been inflated through known spyke DNA), amplified to collect amplicons of variable V3–V4 regions (primers 341F and 805R) of the bacterial 16s rRNA gene and sequenced with MiSeq (2x300bp) Illumina platform.
Contributor(s) Ramazzotti M, Amedei A, Di Gloria L
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Submission date Feb 01, 2024
Last update date Mar 01, 2024
Contact name Matteo Ramazzotti
Organization name University of Florence
Department Experimental and Clinical Biomedical Sciences
Street address viale Morgagni 50
City Firenze
ZIP/Postal code 50134
Country Italy
Platforms (3)
GPL16085 Illumina MiSeq (Escherichia coli)
GPL16417 Illumina MiSeq (Mus musculus)
GPL34159 Illumina MiSeq (human blood metagenome)
Samples (15)
GSM8058751 Reads obtained from sequencing of sample Blood1
GSM8058752 Reads obtained from sequencing of sample Blood3
GSM8058753 Reads obtained from sequencing of sample Blood5
BioProject PRJNA1072063

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Supplementary file Size Download File type/resource
GSE254843_dna-sequences.fasta.gz 31.9 Kb (ftp)(http) FASTA
GSE254843_feature_table.tsv.gz 12.1 Kb (ftp)(http) TSV
GSE254843_taxonomy.txt.gz 10.4 Kb (ftp)(http) TXT
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Processed data are available on Series record

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