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| Status |
Public on Jun 21, 2024 |
| Title |
Transcriptomic response in osteoarthritis determined by single-cell RNA sequencing in a naturally occurring equine model |
| Organism |
Equus caballus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We used single-cell RNA sequencing to characterize the heterogeneity of equine synovial fluid and synovial tissue in a healthy and osteoarthritic joints.
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| Overall design |
The complete dataset consists of 150bp paired end sequencing data obtained using a 10x Genomics single-cell RNA sequencing platform (Chromium Next GEM Single Cell 3สน Kit v3.1 protocol). The final dataset consists of data from 4 biologically distinct horses. Synovial fluid samples were collected from an osteoarthritis (OA) affected joint and the contralateral normal joint for the following library pairs: sf_normal_1+sf_oa_1 and sf_normal_2+sf_oa_2. The remaining two synovial fluid samples were obtained from biologically distinct horses, one from an OA affected joint (sf_oa_3) and one from an unaffected normal joint (sf_normal_3). The final two libraries (syn_normal_1+syn_oa_1) were synovial tissue samples obtained from the same horse from which the sf_normal_2+sf_oa_2 libraries were prepared; syn_normal_1 was collected from the unaffected normal joint while syn_oa_1 was collected from the OA affected joint.
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| Contributor(s) |
Ammons DT, Pezzanite LM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 01, 2024 |
| Last update date |
Jun 21, 2024 |
| Contact name |
Dylan T Ammons |
| E-mail(s) |
Dylan.Ammons@colostate.edu
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| Organization name |
Colorado State University
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| Department |
Clinical Sciences
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| Street address |
200 W Lake St
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| City |
Fort Collins |
| State/province |
CO |
| ZIP/Postal code |
80523 |
| Country |
USA |
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| Platforms (1) |
| GPL26749 |
Illumina NovaSeq 6000 (Equus caballus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1072060 |
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