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Series GSE254550 Query DataSets for GSE254550
Status Public on Apr 18, 2024
Title Cranial photo-immunologic regulation along the skull-meninges-brain axis for promoting recovery from ischemic brain injury
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Stroke involves in the interaction between central and peripheral immune systems. Skull bone marrows serve as reservoirs for immune cells in brain borders, and can rapidly respond to perturbations in the brain environment. Hence, targeting the skull bone marrow to modulate neuroimmune communications along the calvaria-meninges-brain axis would potentially improve stroke prognosis. Here, we successfully achieved cranial immunomodulation via ultraviolet (UV) irradiation of the interparietal region, which was characterized by rich marrow cavities and channels connecting the skull and meninges. Utilizing the recently-developed long-term clearing cranial window that ensured the integrity of skull, we discovered that the cranial photo-immunologic regulation (CPR) could promote cerebrovascular regeneration and aid in neurovascular repair post ischemic stroke. Single-cell transcriptome analysis revealed that meninge could be a crucial neuroimmune interface for ischemic stroke-induced immune responses. And, CPR could restore the stroke-induced alterations in cellular gene expression, especially meningeal B cells. Further we demonstrated that CPR could effectively alleviate the excessive suppression of meningeal B cell activation caused by ischemic stroke. This work opens avenues for immunoregulation through the skull-meninges-brain axis and provides valuable insights for immunomodulatory therapies in brain diseases.
Overall design Single-cell transcriptomic profiling was performed on Sham, tMCAO (transient middle cerebral artery occlusion), and tMCAO+UVB(ultraviolet B) treated mouse skull, meninges, and brain tissue separately.
Contributor(s) Zhang C, Liu C, Zeng J, Wang L, Dai X, Tao T, Feng W
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Submission date Jan 30, 2024
Last update date Apr 18, 2024
Contact name Wei Feng
Organization name Central People's Hospital of Zhanjiang
Street address 236 Yuanzhu Road
City Zhanjiang
ZIP/Postal code 524045
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM8045768 Sham_meninges
GSM8045769 Sham_skull
GSM8045770 tMCAO_meninges
BioProject PRJNA1071029

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GSE254550_RAW.tar 443.7 Mb (http)(custom) TAR (of TAR)
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