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| Status |
Public on Feb 19, 2024 |
| Title |
Gene expression profile at single cell level of human pneumocytes and fibroblasts cultured as organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Alveolar type 2 (AT2) are part of the stem cell niche of the lung and their differentiation is required for pulmonary homeostasis and tissue regeneration. A disturbed crosstalk between fibroblasts and epithelial cells contributes to the loss of lung structure in chronic lung diseases. We used single cell RNA sequencing to analyze the interaction of human fibroblasts and the alveolar epithelium modelled in organoid cultures.
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| Overall design |
Human AT2 cells were seeded in Matrigel in co-culture with or without primary fibroblasts. Single cell suspensions were obtained after 21 days and single cell transcriptome profiling was performed by BD Rhapsody™ Single-Cell Analysis System.
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| Contributor(s) |
Beisswenger C, Ritzmann F, Yao Y, Bals R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 29, 2024 |
| Last update date |
Feb 19, 2024 |
| Contact name |
Felix Ritzmann |
| E-mail(s) |
agbalshomburg@gmail.com
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| Organization name |
Saarland University
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| Street address |
Kirrberger Street
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| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1070587 |
