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| Status |
Public on Sep 19, 2024 |
| Title |
ZmICE1a regulates defense-storage trade-off in maize endosperm [RNA-seq] |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The endosperm of cereal grains feeds the entire world as major food supply, however little is known for its defense responses during endosperm development. Inducer of CBF Expression 1 (ICE1) is a known regulator of cold tolerance in plants. ICE1 has a monocot-specific homologue that is preferentially expressed in cereal grains but with an unclear regulatory function. Here, we characterized the function of monocot-specific ZmICE1 (ZmICE1a) in maize (Zea mays) kernel. mRNA in situ hybridization (ISH) revealed that ZmICE1a is predominantly expressed in the peripheral endosperm (AL, SAL, and BETL). Loss-of-function of ZmICE1a reduced starch content and kernel weight. RNA-seq analysis revealed contradicted expression patterns between starch synthesis genes and genes involved in defense and phytohormone synthesis in zmice1a developing endosperm. Coupled with CUT&Tag-seq analysis, we found that ZmICE1a positively regulates genes in starch synthesis, while negatively regulates genes in AL-specific defense and the synthesis of IAA and JA. Explant assays revealed that exogenous IAA or JA induces the expression of numerous defense genes. mRNA ISH assays revealed that the defense genes induced by IAA and JA, exhibit distinct spatial specific expression in BETL and SAL, respectively. Moreover, we dissected a JA-ZmJAZ9-ZmICE1a-MPI signaling axis involved in JA-mediated defense regulation. Overall, our study revealed ZmICE1a as a key regulatory for endosperm defense responses, and coordinates defense-storage tradeoffs during endosperm development.
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| Overall design |
Identification of ZmICE1a regulatory functions using RNA-seq and CUT&Tag-seq during maize endosperm development
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| Citation missing |
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| Submission date |
Jan 25, 2024 |
| Last update date |
Sep 20, 2024 |
| Contact name |
Wang Qun |
| E-mail(s) |
qunwang@cau.edu.cn
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| Phone |
18101265332
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| Organization name |
China Agricultural University
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| Street address |
West Campus, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing
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| City |
Bei Jing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (1) |
| GPL25410 |
Illumina NovaSeq 6000 (Zea mays) |
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| Samples (24)
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| GSM8035526 |
Mutant ZmICE1a, replicate 1 |
| GSM8035527 |
Mutant ZmICE1a, replicate 2 |
| GSM8035528 |
Mutant ZmICE1a, replicate 3 |
| GSM8035529 |
Wild type_IAA-untreatment, replicate 1 |
| GSM8035530 |
Wild type_IAA-untreatment, replicate 2 |
| GSM8035531 |
Wild type_IAA-untreatment, replicate 3 |
| GSM8035532 |
Wild type_IAA-treatment, replicate 1 |
| GSM8035533 |
Wild type_IAA-treatment, replicate 2 |
| GSM8035534 |
Wild type_IAA-treatment, replicate 3 |
| GSM8035535 |
Wild type_JA-untreatment, replicate 1 |
| GSM8035536 |
Wild type_JA-untreatment, replicate 2 |
| GSM8035537 |
Wild type_JA-untreatment, replicate 3 |
| GSM8035538 |
Wild type_JA-treatment, replicate 1 |
| GSM8035539 |
Wild type_JA-treatment, replicate 2 |
| GSM8035540 |
Wild type_JA-treatment, replicate 3 |
| GSM8035541 |
Mutant_JA-untreatment, replicate 1 |
| GSM8035542 |
Mutant_JA-untreatment, replicate 2 |
| GSM8035543 |
Mutant_JA-untreatment, replicate 3 |
| GSM8035544 |
Mutant_JA-treatment, replicate 1 |
| GSM8035545 |
Mutant_JA-treatment, replicate 2 |
| GSM8035546 |
Mutant_JA-treatment, replicate 3 |
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| Relations |
| BioProject |
PRJNA1068856 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE254177_RAW.tar |
3.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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