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| Status |
Public on Sep 27, 2024 |
| Title |
The effects of HSP60 chaperone deficiency on zebrafish larvae |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To understand the effects of Hsp60 deficiency in developing vertebrates, we generated CRISPR/Cas9-mediated hspd1 knockout zebrafish lines by targeting exon 2 to induce a frameshift mutation. We selected an allele with a 56 base pair deletion inducing a frameshift mutation leading to loss of protein functions. We examined the transcriptome changes in zebrafish larvae at 5 dpf .
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| Overall design |
Wildtype control and hspd1-/- larvae at 5dpf, were analyzed by RNASeq. For this purpose, 3 biological replicates of groups of 5 genotyped larvae from each genotype were pooled and analyzed.
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| Web link |
https://www.clinicalkey.com/#!/content/playContent/1-s2.0-S2212877824001406?returnurl=https:%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2212877824001406%3Fshowall%3Dtrue&referrer=https:%2F%2Fpubmed.ncbi.nlm.nih.gov%2F
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| Citation(s) |
39147275 |
| |
| Submission date |
Jan 24, 2024 |
| Last update date |
Sep 28, 2024 |
| Contact name |
Peter Bross |
| E-mail(s) |
peter.bross@clin.au.dk
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| Phone |
+4578455404
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| Organization name |
Aarhus University and Aarhus University Hospital
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| Department |
Department of Clinical Medicine
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| Lab |
Research Unit for Molecular Medicine (MMF)
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| Street address |
Palle Juul-Jensens Boulevard 99
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| City |
Research Unit for Molecular Medicine (MMF) |
| State/province |
Region Midt |
| ZIP/Postal code |
8200 |
| Country |
Denmark |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1068542 |
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