|
| Status |
Public on Dec 15, 2011 |
| Title |
Genome-wide analysis of the binding pattern of the transcriptional co-activator Cbp. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
The transcriptional coactivator Cbp is critical for hematopoietic stem cell (HSC) development. However, its role in adult HSC and the mechanistic detail of Cbp control of HSC function remain unknown. Using conditional deletion of Cbp in the adult HSC compartment, we demonstrate an altered balance between differentiation and self-renewal with gradual loss of phenotypic HSC, differentiation defects in lower compartments and the development of myeloid malignancies. In addition, we demonstrate that Cbp -/- HSCs reconstitute hematopoiesis with lower efficiency than their wild type counterparts and readily exhaust over time when placed under the replicative stress of serial transplantation. Furthermore, we demonstrate abnormal cell cycle (re)entry and apoptosis in HSC which, with preferential differentiation, also contribute to stem cell exhaustion. Finally we demonstrate global transcriptional abnormalities predicted to alter cell cycle control, balanced differentiation and HSC function upon Cbp deletion and link Cbp to a critical HSC transcriptional regulatory network through genome-wide analysis of Cbp binding.
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| |
|
| Overall design |
1 sample (Cbp) and 1 control, all prepared from a hematopoietic progenitor/stem cell line (BM-HPC)
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| |
|
| Contributor(s) |
Chan W, Hannah RL, Dawson MA, Pridans C, Foster D, Joshi A, Göttgens B, van Deursen J |
| Citation(s) |
22006020 |
| Submission date |
Nov 10, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Wai-In Chan |
| E-mail(s) |
chanwaiin@yahoo.com
|
| Organization name |
Cambridge Institute for Medical Research
|
| Department |
Haematology
|
| Street address |
Wellcome Trust/MRC Building, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0XY |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE25274 |
The transcriptional coactivator Cbp regulates self-renewal and differentiation in adult hematopoietic stem cells |
|
| Relations |
| SRA |
SRP004447 |
| BioProject |
PRJNA142571 |
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