Expression profiling by high throughput sequencing
Summary
To decipher gene expression controlled by the five highly homologous group S1 bZIP transcription factors during the reproductive growth phase of Arabidopsis thaliana, we generated triple (bzip2/-11/-44) and quintuple (bzip1/-2/-11/-44/-53) mutants of these factors using CRISPR/Cas9 and analysed gene expression in distinct C source (source leaves) or C sink (sink leaves, rosette buds, flowers) tissues.
Overall design
We performed RNAseq from 3 biological replicates per genotype (wild type (WT, Arabidopsis ecotype Col-0), triple and quintuple bZIP mutants) and tissue (source leaves, sink leaves, rosette buds, flowers)
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