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| Status |
Public on Sep 11, 2024 |
| Title |
Endothelial Pim3 kinase protects the vascular barrier during lung metastasis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate transcriptome changes of endothelial cells during cancer cell response, we studied HUVEC RNA expression in conditioned medium derived from empty plates versus B16-F10 melanoma cultures.
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| Overall design |
Endothelial cells (ECs) form a tissue-specific barrier for disseminating cancer cells in distant organs. However, the molecular regulation of the ECs in the metastatic niche is poorly understood. Here, we analyzed the transcriptional reprogramming of cultured human ECs (HUVECs) in response to potential signal mediators sectered to the cultured medium by B16-F10 melanoma cells. Melanoma cells were cultured for 48h, after which conditioned medium was collected, spun and added to replace HUVEC culture medium. As a control, HUVEC cultures were treated with conditioned medium stored in similar conditions on empty plates. HUVEC cells were cultured in conditioned medium for 16 h after which total RNA was isolated and processed for mRNA-Seq.
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| Contributor(s) |
Santio N, Kaipainen P, Halme A, Saharinen P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 23, 2023 |
| Last update date |
Sep 11, 2024 |
| Contact name |
Niina Santio |
| E-mail(s) |
niina.santio@helsinki.fi
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| Organization name |
University of Helsinki
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| Department |
Research Program Unit, CAN-PRO
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| Lab |
Saharinen laboratory
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| Street address |
Haartmaninkatu 8
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| City |
Helsinki |
| ZIP/Postal code |
00290 |
| Country |
Finland |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1056275 |
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