|
| Status |
Public on Nov 09, 2010 |
| Title |
Human fetal penile smooth muscle cells (hfPSCM): Control vs PCB mixtures treatments |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The effects exerted by three mixtures of Polychlorinated Biphenyls (PCB), one featuring dioxin-like (DL) and two featuring non dioxin-like (NDL) congeners, on human fetal corpora cavernosa cells representative of a major male endocrine-sensitive organ, have been evaluated by gene expression profiling. PCB congeners concentrations used were derived from human internal exposure data to explore the impact of the adult body burden on a fetal tissue. Cell exposure were performed for 72h mimicking a chronic exposure.
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| Overall design |
Experiment conditions: control, three treatments with PCB-Mix1, PCB-Mix2 or PCB-Mix3. Biological replicates: 3 control replicates, 3 replicates per treatment. Technical replica: 2 (dye swap) per biological replica. Direct comparison design (control vs treated samples) in 12/16 arrays (biological replicates 1 and 2); Loop design in 4/16 arrays (biological replicate 3).
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| Contributor(s) |
Tait S |
| Citation(s) |
21334430 |
| |
| Submission date |
Nov 08, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Paola Di Bonito |
| E-mail(s) |
paola.dibonito@iss.it
|
| Organization name |
Istituto Superiore di SanitÃ
|
| Department |
Dpt. Infectious Diseases
|
| Street address |
Viale Regina Elena 299
|
| City |
Rome |
| State/province |
Italy |
| ZIP/Postal code |
00163 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (16)
|
| GSM618837 |
hfPSMC_CTRLvsMix1_biolrep1_techrepl1 |
| GSM618838 |
hfPSMC_CTRLvsMix2_biolrep1_techrepl1 |
| GSM618839 |
hfPSMC_CTRLvsMix3_biolrep1_techrepl1 |
| GSM618840 |
hfPSMC_CTRLvsMix1_biolrep1_techrepl2 |
| GSM618841 |
hfPSMC_CTRLvsMix2_biolrep1_techrepl2 |
| GSM618842 |
hfPSMC_CTRLvsMix3_biolrep1_techrepl2 |
| GSM618843 |
hfPSMC_CTRLvsMix1_biolrep2_techrepl1 |
| GSM618844 |
hfPSMC_CTRLvsMix2_biolrep2_techrepl1 |
| GSM618845 |
hfPSMC_CTRLvsMix3_biolrep2_techrepl1 |
| GSM618846 |
hfPSMC_CTRLvsMix1_biolrep2_techrepl2 |
| GSM618847 |
hfPSMC_CTRLvsMix2_biolrep2_techrepl2 |
| GSM618848 |
hfPSMC_CTRLvsMix3_biolrep2_techrepl2 |
| GSM618849 |
hfPSMC_CTRLvsMix1_biolrep3_techrepl1 |
| GSM618850 |
hfPSMC_Mix1vsMix2_biolrep3_techrepl2 |
| GSM618851 |
hfPSMC_Mix2vsMix3_biolrep3_techrepl1 |
| GSM618852 |
hfPSMC_CTRLvsMix3_biolrep3_techrepl2 |
|
| Relations |
| BioProject |
PRJNA134299 |
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