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| Status |
Public on Nov 04, 2010 |
| Title |
Transcriptome analysis of Rhizobium etli’s free-living and symbiotic non-growing state. |
| Organism |
Rhizobium etli CFN 42 |
| Experiment type |
Expression profiling by array
|
| Summary |
Gene expression during stationary phase and symbiosis of R. etli CFN42 was compared to that of exponentially growing cells. This allowed us to better understand how R. etli adapts to a non-growing lifestyle, both the free-living and symbiotic state, as well as to determine to what extent this adaptation is similar in both states.
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| Overall design |
R. etli CFN42 was grown at 30˚C in AMS medium supplied with 10 mM NH4Cl and 10 mM succinate while monitoring the optical density (OD) of the culture. Free-living samples were taken at OD600 = 0.3 and 6 hours after reaching the maximum OD, representing early exponential and stationary phase respectively. Bacteroid samples were obtained from nodules 3 weeks after inoculation of Common bean plants (Phaseolus vulgaris cv Limburgse vroege).
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| Contributor(s) |
Vercruysse M, Fauvart M, Michiels J, Cloots L, Engelen K |
| Citation(s) |
21809980 |
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| Submission date |
Nov 03, 2010 |
| Last update date |
Dec 19, 2013 |
| Contact name |
Kristof Engelen |
| Organization name |
KULeuven
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| Lab |
CMPG
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| Street address |
Kasteelpark Arenberg 20
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| City |
Leuven |
| ZIP/Postal code |
3001 |
| Country |
Belgium |
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| Platforms (1) |
| GPL9409 |
Rhizobium etli CFN 42 385K 071203_ReCFN42JM_Tiling |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA134413 |
