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Series GSE250534 Query DataSets for GSE250534
Status Public on Jan 10, 2024
Title Effect of Cabergoline on Post-Lactational Involution and Long-Term Mammary Gland Behavior.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The aim of this study is to assess the impact of cabergoline on the intensity of short-term post-lactational involution and to explore long-term alterations in the epithelial dynamics of the glandular component in Brca1/P53-deficient mice. This study also delves into the transcriptomic changes associated with these processes. To further investigate the molecular changes occurring at these temporal points, RNA sequencing (RNA-seq) was conducted 1 day after post-lactation involution and again at 60 days.
 
Overall design Multiparous Brca1/P53-deficient mice (Liu et al., 2007) with two pregnancies were administered intraperitoneal (IP) cabergoline at a dose of X on the same day of the second weaning. To study the short-term effect of cabergoline, organoids were generated 24 hours later, RNA was extracted, and RNA-seq (Novogene) was performed. Mice treated with cabergoline were compared with untreated controls. For the long-term effect study, the experiment was repeated in another group of mice. Organoids were generated 60 days post-weaning, including multiparous mice treated and untreated with cabergoline and age-equivalent nulliparous mice.
 
Contributor(s) García-Sancha N, Corchado-Cobos R, Blanco-Gómez A, Alonso-López D, de las Rivas J, Pérez-Losada J
Citation(s) 38405932
Submission date Dec 19, 2023
Last update date Mar 06, 2024
Contact name Jesús Pérez Losada
E-mail(s) jperezlosada@usal.es
Organization name Inst. de Inv. Biomédica de Salamanca (IBSAL). H. U. de Salamanca. Inst. de Biología Molecular y Celular del Cáncer (IBMCC).
Department Centro de inv. del Cáncer (CIC).
Street address Campus Miguel de Unamuno
City Salamanca
ZIP/Postal code E37007
Country Spain
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (25)
GSM7981117 Brca1/P53-Deficient Mammary Gland Organoids from Age-Matched Nulliparous Mice 1 [A1_Nulip ]
GSM7981118 Brca1/P53-Deficient Mammary Gland Organoids from Age-Matched Nulliparous Mice 2 [A2_Nulip ]
GSM7981119 Brca1/P53-Deficient Mammary Gland Organoids from Age-Matched Nulliparous Mice 3 [A3_Nulip]
Relations
BioProject PRJNA1054495

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Supplementary file Size Download File type/resource
GSE250534_TPMs.xlsx 6.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA

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