|
| Status |
Public on Jan 13, 2024 |
| Title |
Transcriptome analysis of pulsed activation of MAPK signaling in HEK-RAF-ER cells [RNA_seq_pulse_time_series] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we performed pulsed activation of MAPK signalling by inducing RAF activity of the RAF-ER constract by tamoxifen, and subsequently blocking MAPK activity using MEK inhibitor at different times. Transcriptomes were sampled 0.5 h to 8h after induction
|
| |
|
| Overall design |
Tamoxifen (4OHT, 0.5uM) was induced at t=0h, and the MEK inhibitor (U01126, 20uM) was added after 0.5h, 1h, 2h, and 4h. Transcriptomes were sampled at 0.5h, 1h, 2h, and 4h.
|
| |
|
| Contributor(s) |
Uhlitz F, Sieber A, Blüthgen N |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 19, 2023 |
| Last update date |
Jan 15, 2024 |
| Contact name |
Nils Blüthgen |
| E-mail(s) |
nils.bluethgen@charite.de
|
| Organization name |
Charite Universitätsmedizin Berlin
|
| Department |
Institute of Pathology
|
| Street address |
Chariteplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA1054483 |
Less...
More...