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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 30, 2024 |
| Title |
Muscle-resident mesenchymal progenitors sense and repair peripheral nerve injury via the GDNF-BDNF axis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Fibro-adipogenic progenitors (FAPs) are muscle-resident mesenchymal progenitors that can contribute to muscle tissue homeostasis and regeneration, as well as postnatal maturation and lifelong maintenance of the neuromuscular system. Recently, traumatic injury to the peripheral nerve was shown to activate FAPs, suggesting that FAPs can respond to nerve injury. However, questions of how FAPs can sense the anatomically distant peripheral nerve injury and whether FAPs can directly contribute to nerve regeneration remained unanswered. Here, utilizing single-cell transcriptomics and mouse models, we discovered that a subset of FAPs expressing GDNF receptors Ret and Gfra1 can respond to peripheral nerve injury by sensing GDNF secreted by Schwann cells. Upon GDNF sensing, this subset becomes activated and expresses Bdnf. FAP-specific inactivation of Bdnf (Prrx1-Cre; Bdnf-fl/fl) resulted in delayed nerve regeneration owing to defective remyelination, indicating that GDNF-sensing FAPs play an important role in the remyelination process during peripheral nerve regeneration. In aged mice, significantly reduced Bdnf expression in FAPs was observed upon nerve injury, suggesting the clinical relevance of FAP-derived BDNF in the age-related delays in nerve regeneration. Collectively, our study revealed the previously unidentified role of FAPs in peripheral nerve regeneration, and the molecular mechanism behind FAPs’ response to peripheral nerve injury.
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| Overall design |
Single cell RNA-sequencing analysis was performed using FACS-sorted FAPs from limb muscles below knee after either sciatic nerve crush injury or denervation, at days 3, 7, 14, and 28 post injury, using 12-week-old B6 mice. An uninjured control was also sampled for analysis.
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| Contributor(s) |
Yoo K, Kong Y |
| Citation(s) |
39324575 |
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| Submission date |
Dec 18, 2023 |
| Last update date |
Oct 10, 2024 |
| Contact name |
Kyusang Yoo |
| E-mail(s) |
kpy619@snu.ac.kr
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| Organization name |
Seoul National University
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| Department |
Biological Sciences Department
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| Lab |
Laboratory of Development and Disease
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| Street address |
1 Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1054070 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE250436_RAW.tar |
171.8 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
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