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Series GSE2503 Query DataSets for GSE2503
Status Public on Jun 30, 2006
Title Novel Molecular Markers for non-melanoma skin cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Summary normal skin (no), actinic keratosis (ak), and squamous cell carcinoma (scc) of the skin were examined:

Carcinogenesis is a multi-step process indicated by several genes up- or down-regulated during tumor progression. The type and number of genes involved in non-melanoma skin cancer (NMSC) are still unclear. This study examined and identified different expressed genes in NMSC. Fifteen snap-frozen biopsies of five immunosuppressed organ-transplanted recipients each normal skin, actinic keratosis (AK) and invasive squamous cell carcinoma (SCC) were analysed. Total RNA was extracted reverse transcribed and biotin-labeled cRNA was used for hybridization with Affymetrix HG-U133A gene expression cDNA-microarrays containing 22,283 known genes. Confocal scanner evaluated the signals twice and data analysis was performed by predicition analysis of microarrays (PAM) using nearest shrunken centroids with the threshold 3.5 to identify genes best characterizing normal skin and skin cancer. ANOVA analysis was performed as a second independent method to identify differentially expressed genes (p<0.05, and p<0.15). Quantitative verification of 13 up- or down-regulated genes was performed by real-time RT-PCR and genes were confirmed by sequencing. Broad coherent patterns in normal skin vs. AK and SCC were observed for 118 genes. Some of the genes (e.g. annexin, lamin, and metalloproteinases) up-regulated in carcinomas in our study have previously reported as over-expressed in NMSC. In total 42 genes were up-regulated and 76 genes were down-regulated in non-melanoma skin cancer. The majority of genes identified have not been previously reported different expressed during non-melanoma skin carcinogenesis.
Keywords: ordered
Contributor(s) Nindl I, Dang C, Schmook T, Kuban R, Meyer T, Sterry W, Stockfleth E
Citation(s) 16893473, 20231500
Submission date Apr 06, 2005
Last update date Aug 10, 2018
Contact name Ingo Nindl
Phone +49-30-450518269
Fax +49-30-450518969
Organization name Skin Cancer Center Charite
Department Dermatology
Lab Molecularbiology
Street address Schumannstr. 20/21
City Berlin
ZIP/Postal code 10117
Country Germany
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (15)
GSM47612 cDNA ak 03
GSM47613 cDNA ak 04
GSM47614 cDNA ak 09+15
BioProject PRJNA92029

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