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| Status |
Public on Apr 07, 2024 |
| Title |
Antibacterial activities of the algal bromophenol methylrhodomelol against Pseudomonas aeruginosa |
| Organism |
Pseudomonas aeruginosa |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Methylrhodomelol (1) is a bromophenol from the red alga Vertebrata lanosa (L.) T.A.Christensen that has been associated with antimicrobial properties. Aim of the current study was therefore, to assess the antimicrobial potential of this compound in more detail against the gram-negative pathogen Pseudomonas aeruginosa. 1 exerted weak bacteriostatic activity against different strains when grown in minimal medium, whereas other phenolics were inactive. In addition, 1 (35 and 10 µg/mL) markedly enhanced the susceptibility of multidrug resistant P. aeruginosa towards the aminoglycoside gentamicin, while it did not affect the viability of Vero kidney cells up to 100 µM. Finally, pyoverdine release was reduced in bacteria treated at sub-inhibitory concentration, but no effect on other virulence factors was observed. Transcriptome analysis of treated versus untreated P. aeruginosa indicated an interference of 1 with bacterial carbon and energy metabolism, which was corroborated by RT-qPCR and decreased ATP-levels in treated bacteria.
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| Overall design |
Comparative gene expression analysis of untreated versus treated (methylrhodomelol 75 µM) bacteria at one timepoint.
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| Contributor(s) |
Jacobtorweihen J, Hartmann A, Hofer S, Spiegler V |
| Citation(s) |
38580306 |
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| Submission date |
Dec 06, 2023 |
| Last update date |
Jul 07, 2024 |
| Contact name |
Verena Spiegler |
| Organization name |
University of Münster
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| Street address |
Corrensstraße 48
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| City |
Münster |
| ZIP/Postal code |
48149 |
| Country |
Germany |
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| Platforms (1) |
| GPL33271 |
NextSeq 2000 (Pseudomonas aeruginosa) |
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| Samples (6)
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| GSM7948770 |
Pseudomonas aeruginosa, untreated, rep 1 |
| GSM7948771 |
Pseudomonas aeruginosa, untreated, rep 2 |
| GSM7948772 |
Pseudomonas aeruginosa, untreated, rep 3 |
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| Relations |
| BioProject |
PRJNA1049368 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE249483_diff_genes_deseq2_results_MR_vs_UC_all_genes_annotation.txt.gz |
292.2 Kb |
(ftp)(http) |
TXT |
| GSE249483_raw_read_counts_MR_UC.txt.gz |
67.2 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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