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Series GSE249450 Query DataSets for GSE249450
Status Public on Dec 06, 2023
Title Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation
Organism Bacillus subtilis
Experiment type Expression profiling by high throughput sequencing
Other
Summary The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
Web link https://doi.org/10.1038/s41467-024-51654-6
 
Contributor(s) Iwańska O, Latoch P, Kopik N, Kovalenko M, Lichocka M, Serwa R, Starosta AL
Citation(s) 39169056
Submission date Dec 05, 2023
Last update date Aug 22, 2024
Contact name Agata L. Starosta
E-mail(s) agata.starosta@ibb.waw.pl
Organization name Institute of Biochemistry and Biophysics Polish Academy of Sciences
Lab Laboratory of Translatomics
Street address Pawińskiego 5a
City Warsaw
ZIP/Postal code 02-106
Country Poland
 
Platforms (1)
GPL24109 Illumina NextSeq 500 (Bacillus subtilis)
Samples (64)
GSM7947710 WT 0h, prior to sporulation induction, replicate 1
GSM7947711 WT 0h, prior to sporulation induction, replicate 2
GSM7947712 WT 1h post sporulation induction, replicate 1
This SuperSeries is composed of the following SubSeries:
GSE249448 Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]
GSE249449 Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]
Relations
BioProject PRJNA1049044

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