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| Status |
Public on Mar 27, 2024 |
| Title |
Transcriptome analysis during VCD exposure-induced POI in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
4-vinylcyclohexene diepoxide (VCD) is a reproductively toxic environmental pollutant that causes follicular failure leading to POIs, which can seriously affect a woman's physical health and fertility. Investigating its pathogenic mechanisms can help provide guidance for the prevention of ovarian impairment and the treatment of POI. We built a mouse model of POI by intraperitoneal injection of VCD into female C57BL/6 mice for 15 days. Then compared it with the control group at two time points, day 15 and day 30, including the comparison of phenotypic characteristics and differences in the transcriptome. We performed a comprehensive analysis of the differential genes identified and validated some key genes by RT-PCR. The results showed that sex hormone levels, follicle number and estrous cycle of VCD-induced POI mice were significantly affected at both day 15 and day 30. Regarding DEGs and enrichment results, the results obtained at day 15 were not as significant as those at day 30. Our results provide a preliminary indication that steroid hormone synthesis, DNA damage repair, and impaired oocyte mitosis play an important part in the process by which VCD affects ovarian function. Maybe it was due to impaired follicular development caused by VCD damage to the primordial follicular pool, and with the progression of time, the ovarian damage was aggravated, and it was gradually difficult to perform normal function.
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| Overall design |
We built a mouse model of POI by intraperitoneal injection of VCD into female C57BL/6 mice for 15 days. Then compared it with the control group at two time points, day 15 and day 30, including the comparison of phenotypic characteristics and differences in the transcriptome. We performed a comprehensive analysis of the differential genes identified and validated some key genes by RT-PCR.
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| Contributor(s) |
Li Y, He R, Qin X, Zhu Q, Ma L, Liang X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 01, 2023 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Yi Li |
| E-mail(s) |
liy21@lzu.edu.cn
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| Phone |
18996460417
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| Organization name |
Lanzhou University
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| Street address |
甘肃省兰州市城关区东岗西路1号兰州大学第一医院
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| City |
兰州市 |
| State/province |
甘肃 |
| ZIP/Postal code |
700030 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (15)
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| GSM7927525 |
Ovary, control, rep 4 |
| GSM7927526 |
Ovary, control, rep 5 |
| GSM7927527 |
ovary, VCD affected for 15 days, rep 1 |
| GSM7927528 |
ovary, VCD affected for 15 days, rep 2 |
| GSM7927529 |
ovary, VCD affected for 15 days, rep 3 |
| GSM7927530 |
ovary, VCD affected for 15 days, rep 4 |
| GSM7927531 |
ovary, VCD affected for 15 days, rep 5 |
| GSM7927532 |
ovary, VCD affected for 30 days, rep 1 |
| GSM7927533 |
ovary, VCD affected for 30 days, rep 2 |
| GSM7927534 |
ovary, VCD affected for 30 days, rep 3 |
| GSM7927535 |
ovary, VCD affected for 30 days, rep 4 |
| GSM7927536 |
ovary, VCD affected for 30 days, rep 5 |
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| Relations |
| BioProject |
PRJNA1047560 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE249151_gene_count.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE249151_gene_fpkm.txt.gz |
3.9 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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