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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 19, 2024 |
| Title |
Effect of SMARCA4 knockdown on senescent IMR90 ER:RAS cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the analysis of RNA sequencing aimed at understanding the effects induced by the knockdown of various target genes on senescent cells. These genes were initially identified through a genetic screening process designed to locate regulators responsible for upregulating SASP. Specifically, we focus on exploring the repercussions of SMARCA4 knockdown, a constituent of the SWI/SNF complex subunit, on the SASP and immune responses within senescent cells. The findings will help characterise the role of SMARCA4 inhibition on senescence surveillance by Natural Killer cells.
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| Overall design |
IMR90 ER:RAS cells were transfected with siRNAs against target genes and non-targeting scramble controls (by reverse transfection) one day prior to senescence induction. Oncogene-induced senescence was induced following the administration of 4OHT (tamoxifin) treatment. RNA from the cells was extracted seven days following senescence induction.
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| Contributor(s) |
Reen V, D'Ambrosio M, Gil J, Khadayate S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 30, 2023 |
| Last update date |
Sep 19, 2024 |
| Contact name |
LMS Bioinformatics Core |
| E-mail(s) |
bioinformatics@lms.mrc.ac.uk
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| Organization name |
MRC London Institute of Medical Sciences
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| Department |
Bioinformatics Core
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| Street address |
Hammersmith Hospital Campus, Du Cane Road
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| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (29)
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| GSM7925797 |
4OHT + siSMARCA4_2, 2V |
| GSM7925798 |
4OHT + siSMARCA4_4, 2V |
| GSM7925799 |
DMSO Control + siNT 2, 3V |
| GSM7925800 |
DMSO Control + siNT 4, 3V |
| GSM7925801 |
4OHT Control + siNT 2, 3V |
| GSM7925802 |
4OHT Control + siNT 4, 3V |
| GSM7925803 |
4OHT + siSMARCA4_2, 3V |
| GSM7925804 |
4OHT + siSMARCA4_4, 3V |
| GSM7925805 |
DMSO Control + siNT 2, 1V |
| GSM7925806 |
DMSO Control + siNT 4, 1V |
| GSM7925807 |
4OHT Control + siNT 2, 1VM |
| GSM7925808 |
4OHT Control + siNT 4, 1VM |
| GSM7925809 |
4OHT + siSMARCA4_2, 1V |
| GSM7925810 |
4OHT + siSMARCA4_4, 1V |
| GSM7925811 |
DMSO Control + siNT 2, 2VM |
| GSM7925812 |
DMSO Control + siNT 4, 2VM |
| GSM7925813 |
4OHT Control + siNT 2, 2VM |
| GSM7925814 |
4OHT Control + siNT 4, 2VM |
| GSM7925815 |
4OHT + siSMARCA4_2, 2VM |
| GSM7925816 |
4OHT + siSMARCA4_4, 2VM |
| GSM7925817 |
DMSO Control + siNT 2, 3VM |
| GSM7925818 |
DMSO Control + siNT 4, 3VM |
| GSM7925819 |
4OHT Control + siNT 2, 3VM |
| GSM7925820 |
4OHT Control + siNT 4, 3VM |
| GSM7925821 |
4OHT + siSMARCA4_2, 3VM |
| GSM7925822 |
4OHT + siSMARCA4_4, 3VM |
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| Relations |
| BioProject |
PRJNA1047105 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE249064_rawcounts.csv.gz |
983.3 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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