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| Status |
Public on Dec 04, 2023 |
| Title |
The single-cell transcriptomic atlas and RORA-mediated 3D epigenomic remodeling in driving corneal epithelial differentiation [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Proper differentiation of corneal epithelial cells (CECs) from limbal stem/progenitor cells (LSCs) is required for maintaining ocular homeostasis and clear vision. Here, using a single-cell transcriptomic atlas, we delineate the comprehensive and refined molecular regulatory dynamics during human CEC development and differentiation. We find that RORA is a CEC-specific molecular switch that initiates and drives LSCs to differentiate into mature CECs by activating PITX1. RORA dictates CEC differentiation by establishing CEC-specific enhancers and chromatin interactions between CEC gene promoters and distal regulatory elements. Conversely, RORA silences LSC-specific promoters and disrupts promoter-anchored chromatin loops to turn off LSC genes. Collectively, our work provides detailed and comprehensive insights into the transcriptional dynamics and RORA-mediated epigenetic remodeling underlying human corneal epithelial differentiation.
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| |
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| Overall design |
ChIP-seq for RORA-flag, H3K27ac, H3K27me3 and H3K4me3 in RORA-overexpressed LSCs. ChIP-seq for H3K27ac, H3K27me3 and H3K4me3 in control LSCs.
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| |
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| Citation(s) |
38177186 |
| |
| Submission date |
Nov 29, 2023 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Mingsen Li |
| E-mail(s) |
lims3@mail2.sysu.edu.cn
|
| Organization name |
Sun Yat-sen University
|
| Department |
Zhongshan Ophthalmic Center
|
| Street address |
No. 7 Jinsui road Tianhe district
|
| City |
Guanzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE249150 |
The single-cell transcriptomic atlas and RORA-mediated 3D epigenomic remodeling in driving corneal epithelial differentiation |
|
| Relations |
| BioProject |
PRJNA1046467 |
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