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| Status |
Public on Mar 06, 2024 |
| Title |
The germline regulates longevity and somatic repair in a sex-specific manner (RNA-Seq) |
| Organism |
Nothobranchius furzeri |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Classical evolutionary theories propose tradeoffs between reproduction, damage repair, and lifespan. However, the specific role of the germline in shaping vertebrate aging remains largely unknown. Here, we use the turquoise killifish (N. furzeri) to genetically arrest germline differentiation at discrete stages, and examine how different ‘flavors’ of infertility impact life-history. We first constructed a comprehensive single-cell gonadal atlas, providing cell-type-specific markers for downstream phenotypic analysis. Next, investigating our genetic models revealed that only germline ablation enhanced female damage repair, while arresting germline differentiation did not. Conversely, germline-ablated males were significantly long-lived, indicating that the mere presence of the germline can negatively affect lifespan. Transcriptomic analysis highlighted enrichment of pro-longevity pathways and genes, with functional conservation in germline-ablated C. elegans. Finally, germline depletion extended male healthspan through rejuvenated metabolic functions. Our results suggest that different germline manipulation paradigms can yield pronounced sexually dimorphic phenotypes, implying alternative mechanisms to classical evolutionary tradeoffs.
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| Overall design |
Livers from killifish were used, and each group varied by sex (males or females), age (young or old), and genotype (wild-type or dnd1 mutant).
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| Contributor(s) |
Eitan M, Atlan T, Sun X, Marinov G, Greenleaf WJ, Ram O, Harel I |
| Citation(s) |
38750187 |
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| Submission date |
Nov 27, 2023 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Itamar Harel |
| E-mail(s) |
itamarh@mail.huji.ac.il
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| Organization name |
The Hebrew University of Jerusalem
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| Department |
Genetics
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| Lab |
Harel
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| Street address |
Givat Ram
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| City |
Jerusalem |
| ZIP/Postal code |
9190401 |
| Country |
Israel |
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| Platforms (1) |
| GPL33195 |
NextSeq 2000 (Nothobranchius furzeri) |
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| Samples (28)
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| GSM7919296 |
Male, WT, old sample 15 |
| GSM7919297 |
Male, WT, old sample 16 |
| GSM7919298 |
Male, WT, old sample 17 |
| GSM7919299 |
Male, WT, old sample 18 |
| GSM7919300 |
Female, WT, young sample 22 |
| GSM7919301 |
Female, WT, young sample 6 |
| GSM7919302 |
Female, WT, young sample 7 |
| GSM7919303 |
Female, WT, young sample 8 |
| GSM7919304 |
Female, WT, old sample 10 |
| GSM7919305 |
Female, WT, old sample 11 |
| GSM7919306 |
Female, WT, old sample 9 |
| GSM7919307 |
Male, dnd1 mutant, young sample 2 |
| GSM7919308 |
Male, dnd1 mutant, young sample 25 |
| GSM7919309 |
Male, dnd1 mutant, young sample 27 |
| GSM7919310 |
Male, dnd1 mutant, young sample 28 |
| GSM7919311 |
Male, dnd1 mutant, old sample 19 |
| GSM7919312 |
Male, dnd1 mutant, old sample 20 |
| GSM7919313 |
Male, dnd1 mutant, old sample 24 |
| GSM7919314 |
Female, dnd1 mutant, young sample 1 |
| GSM7919315 |
Female, dnd1 mutant, young sample 21 |
| GSM7919316 |
Female, dnd1 mutant, young sample 26 |
| GSM7919317 |
Female, dnd1 mutant, old sample 12 |
| GSM7919318 |
Female, dnd1 mutant, old sample 13 |
| GSM7919319 |
Female, dnd1 mutant, old sample 14 |
| GSM7919320 |
Female, dnd1 mutant, old sample 23 |
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| This SubSeries is part of SuperSeries: |
| GSE248741 |
The germline regulates longevity and somatic repair in a sex-specific manner |
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| Relations |
| BioProject |
PRJNA1045687 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248739_matrix_expression_RNAseq_star2306.csv.gz |
961.6 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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