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Series GSE248690 Query DataSets for GSE248690
Status Public on Jan 31, 2024
Title Gut bacteria-derived serotonin promotes immune tolerance in early life through regulation of neonatal gut T cells I
Organism Mus musculus
Experiment type Other
Summary The gut microbiota promotes immune system development in early life, but the interactions between the gut metabolome and immune cells in the neonatal gut remains largely undefined. Here, we demonstrate that the neonatal gut is uniquely enriched with neurotransmitters, including serotonin; specific gut bacteria produce serotonin directly while downregulating monoamine oxidase A to limit serotonin breakdown. Serotonin directly signals to T cells to increase intracellular indole-3-acetaldehdye to inhibit mTOR activation and thereby promotes the differentiation of regulatory T cells, both ex vivo and in vivo in the neonatal intestine. Oral gavage of serotonin into neonatal mice leads to long-term T cell-mediated antigen-specific immune tolerance towards both dietary antigens and commensal bacteria. Together, our study has uncovered an important role for unique gut bacteria to increase serotonin availability in the neonatal gut and a novel function of gut serotonin to shape T cell response to dietary antigens and commensal bacteria to promote immune tolerance in early life.
Overall design Small intestine luminal contents were harvested from adult (8 weeks) and neonatal (14 days) mice. DNA was extracted from luminal contents and the V4-V5 region of the 16S rRNA gene was amplified using universal primers and sequenced on an Illumina MiSeq sequencer.
Contributor(s) Sanidad KZ, Rager SL, Carrow HC, Ananthanarayanan A, Callaghan R, Hart LR, Li T, Ravisankar P, Brown JA, Amir M, Jin JC, Savage AR, Luo R, Rowdo FM, Martin ML, Silver RB, Guo C, Krumsiek J, Inohara N, Zeng MY
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Submission date Nov 27, 2023
Last update date Jan 31, 2024
Contact name Katherine Sanidad
Organization name Weill Cornell Medicine
Street address 413 E69th St
City New York
ZIP/Postal code 11365
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM7918805 Adult_1
GSM7918806 Adult_2
GSM7918807 Adult_3
This SubSeries is part of SuperSeries:
GSE248694 Gut bacteria-derived serotonin promotes immune tolerance in early life through regulation of neonatal gut T cells
BioProject PRJNA1045602

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Supplementary file Size Download File type/resource
GSE248690_16S_processed_F1.xlsx 789.6 Kb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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