 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Nov 22, 2023 |
| Title |
Tumor cell-intrinsic p38 MAPK signaling promotes IL1⍺-mediated stromal inflammation and therapeutic resistance in pancreatic cancer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pancreatic ductal adenocarcinoma (PDAC) is characterized by a KRAS-driven inflammatory program and a desmoplastic stroma, which contribute to the profoundly chemoresistant phenotype. The tumor stroma contains an abundance of cancer-associated fibroblasts (CAFs), which engage in extensive paracrine crosstalk with tumor cells to perpetuate pro-tumorigenic inflammation. Interleukin-1α (IL1α), a pleiotropic, tumor cell-derived cytokine, plays a critical role in shaping the stromal landscape. We have identified p38 MAPK as a key regulator of IL1α gene expression and utilized single-cell RNA sequencing to study the role of p38 MAPK in PDAC
|
| |
|
| Overall design |
Single-cell RNA sequencing of tumors derived from genetically-engineered Ptf1aCre/+; LSL-KrasG12D/+; Tgfbr2flox/flox (PKT) mice treated with either a p38 MAPK inhibitor (ARRY-614) or control vehicle. For 2.5 weeks, three PKT mice were treated with ARRY-614, and three PKT mice were treated with control vehicle. Upon sacrifice, tumors were harvested and three tumors from each treatment group were pooled for downstream sequencing. Single-cell RNA sequencing was performed using the 10X Genomics platform.
|
| |
|
| Contributor(s) |
Singh SP, Dosch AR, Mehra S, De Castro Silva I, Bianchi A, Tonin-Garrido V, Zhou Z, Adams A, Amirian H, Box EW, Sun X, Ban Y, Datta J, Nagathihalli NS, Merchant NB |
| Citation(s) |
38285896 |
| |
| Submission date |
Nov 16, 2023 |
| Last update date |
Feb 24, 2024 |
| Contact name |
Nipun B Merchant |
| E-mail(s) |
nmerchant@med.miami.edu
|
| Organization name |
University of Miami/Jackson Memorial Hospital - Miami, FL
|
| Department |
Surgery
|
| Street address |
1550 Northwest 10th Avenue, Fox 140
|
| City |
Miami |
| State/province |
Florida |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1041392 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE248003_RAW.tar |
191.1 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
