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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2011 |
| Title |
Expression data from WT, Crebbp+/- and Ep300+/- bone marrow stroma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We found that the bone marrow microenvironment of Crebbp+/- mice was unable to properly maintain the immature stem - and progenitor pools. Instead, it stimulates myeloid differentiation that progresses into a myeloproliferative-like disease. Since CREBBP is a transcriptional co-activator, we used gene expression analysis to globally assess functional deficiencies in Crebbp+/- bone marrow stroma cells at a molecular level. Ep300 encodes a protein which is highly similar in structure and function to CREBBP; nevertheless, Ep300+/- mice suffer neither excessive myeloid differentiation nor loss of HSCs. Therefore, to identify expression changes specifically related to Crebbp heterozygosity, we focused on genes that showed significant differences in expression levels between Crebbp+/- and wild-type bone marrow stroma but no difference between Ep300+/- and wild-type.
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| Overall design |
Bone marrow stroma was established from wild-type, Crebbp+/- and Ep300+/- mice that were 3-4 months old for RNA extraction and hybridization on Affymetrix microarrays. There are 4 biological replicates for each genotype used.
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| Contributor(s) |
Rebel V |
| Citation(s) |
21555743 |
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| Submission date |
Oct 18, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Vivienne Rebel |
| Organization name |
UTHSCSA
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| Street address |
8403 Floyd Curl Drive
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| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78229 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM610238 |
BM stroma Wildtype, biological rep1 |
| GSM610239 |
BM stroma Wildtype, biological rep2 |
| GSM610240 |
BM stroma Wildtype, biological rep3 |
| GSM610241 |
BM stroma Wildtype, biological rep4 |
| GSM610242 |
BM stroma Crebbp+/-, biological rep1 |
| GSM610243 |
BM stroma Crebbp+/-, biological rep2 |
| GSM610244 |
BM stroma Crebbp+/-, biological rep3 |
| GSM610245 |
BM stroma Crebbp+/-, biological rep4 |
| GSM610246 |
BM stroma Ep300+/-, biological rep1 |
| GSM610247 |
BM stroma Ep300+/-, biological rep2 |
| GSM610248 |
BM stroma Ep300+/-, biological rep3 |
| GSM610249 |
BM stroma Ep300+/-, biological rep4 |
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| Relations |
| BioProject |
PRJNA132219 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24773_RAW.tar |
43.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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