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| Status |
Public on Nov 18, 2023 |
| Title |
A study on the impact of the absence of the NIP or NrpR gene on gene expression of Streptococcus salivarius K12 (SAL) |
| Organism |
Streptococcus salivarius K12 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
NIP and NrpR forms a pair for the transcriptional regulator in Streptococcus salivarius K12 (SAL). To explore the target genes regulated by the NIP-NrpR pair in Streptococcus salivarius K12 (SAL), we generated mutants in which the NIP or NrpR gene has been knocked down through complement recombination. Comparative transcriptome profiling of WT, â–²nrpR, and nip* mutant strains revealed that the sar BGC is the major regulatory target controlled by the NIP signaling pathway in SAL.
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| Overall design |
We then performed gene expression profiling analysis using data obtained from RNA-seq of 3 different samples at the exponential growth phase of SAL. Comparative gene expression profiling analysis of RNA-seq data between wild type and mutant, total RNA was isolated from 3 independent growths (3 biological replicates)
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| Contributor(s) |
Do H, Makthal N, Kumaraswami M |
| Citation(s) |
38228859 |
| |
| Submission date |
Nov 13, 2023 |
| Last update date |
Feb 15, 2024 |
| Contact name |
Muthiah Kumaraswami |
| E-mail(s) |
mkumaraswami@houstonmethodist.org
|
| Phone |
713-441-5881
|
| Organization name |
Houston Methodist Research Institute
|
| Lab |
KUMARASWAMI LAB
|
| Street address |
6670 Bertner Avenue
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL33931 |
NextSeq 2000 (Streptococcus salivarius K12) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1039769 |
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