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| Status |
Public on May 15, 2024 |
| Title |
Transcriptomic characterization of circulating neutrophils isolated from patients with metabolic dysfunction-associated steatohepatitis (MASH), formerly known as nonalcoholic steatohepatitis (NASH). |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Neutrophils, a major innate immune cell population, are the most abundant circulating white blood cell in humans. They play a crucial role in host defense against infection; however, aberrant neutrophil activation may induce tissue damage via sterile inflammation. Neutrophil accumulation has been identified as a feature of the inflammatory response observed MASH and has been associated with liver fibrosis and cirrhosis. In the present study, we used RNA-Seq analysis of purified neutrophils to characterize their dysregulation in MASH.
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| Overall design |
Using negative selection, we isolated peripheral neutrophils from subjects with biopsy proven mild and sever MASH and control individuals. Total RNA was extracted, DNase treated, and differential gene expression was assessed using next generation RNA sequencing (RNA-Seq) techniques.
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| Contributor(s) |
Maretti-Mira AC, Salomon MP, Golden-Mason L |
| Citation(s) |
38791066 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 DK117004 |
Innate Immunity, Cholesterol, and NASH Pathogenesis |
UNIVERSITY OF SOUTHERN CALIFORNIA |
Lucy Mary Golden |
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| |
| Submission date |
Nov 10, 2023 |
| Last update date |
May 28, 2024 |
| Contact name |
Ana C Maretti-Mira |
| E-mail(s) |
anamaretti@outlook.com
|
| Organization name |
USC
|
| Department |
Medicine
|
| Lab |
Golden Lab
|
| Street address |
1450 Biggy Street, NRT 4516
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1038739 |
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