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| Status |
Public on Nov 12, 2023 |
| Title |
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase [easyCLIP] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
During meiosis, germ cell and stage-specific components impose additional layers of regulation on the core cell cycle machinery to set up an extended G2 period termed meiotic prophase. In Drosophila males, meiotic prophase lasts 3.5 days, during which spermatocytes upregulate over 1800 genes and grow 25-fold. Previous work has shown that the cell cycle regulator Cyclin B (CycB) is subject to translational repression in immature spermatocytes, mediated by the RNA-binding protein Rbp4 and its partner Fest. Here, we show that the spermatocyte-specific protein Lut is required for translational repression of cycB in an 8-h window just before spermatocytes are fully mature. In males mutant for rbp4 or lut, spermatocytes enter and exit meiotic division 6-8 h earlier than in wild type. In addition, spermatocyte-specific isoforms of Syncrip (Syp) are required for expression of CycB protein in mature spermatocytes and normal entry into the meiotic divisions. Lut and Syp interact with Fest independent of RNA. Thus a set of spermatocyte-specific regulators choreograph the timing of expression of CycB protein during male meiotic prophase.
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| Overall design |
Libraries were made following the protocol: Ultraviolet (UV) cross-linked and immunoprecipitation (CLIP)-sequencing (easyCLIP, Porter et al., 2021). easyCLIP was performed on testes from eYFP-Syp males as well as wild-type testes with no epitope (w1118, negative control), and cDNA libraries were made from the recovered RNAs.
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| Contributor(s) |
Matias NR, Porter DF |
| Citation(s) |
37882771 |
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| Submission date |
Nov 09, 2023 |
| Last update date |
Jan 03, 2024 |
| Contact name |
Neuza Reis Matias |
| E-mail(s) |
nmatias@stanford.edu
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| Organization name |
Stanford University
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| Department |
Developmental Biology
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| Lab |
Margaret T. Fuller
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| Street address |
279 Campus Drive
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE247388 |
Cell-type-specific interacting proteins collaborate to regulate the timing of Cyclin B protein expression in male meiotic prophase |
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| Relations |
| BioProject |
PRJNA1037701 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE247385_RAW.tar |
20.9 Mb |
(http)(custom) |
TAR (of BW, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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