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| Status |
Public on Oct 31, 2023 |
| Title |
Expression data from mouse rGAS6-stimulated BMDMs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To determine the mechanism by which ATF3 regulated the survival/proliferation of MerTK+ cardiac macrophages, microarray was performed to identify gene expression differences in MerTK+ macrophages from WT and ATF3-CKO mice under the HR state.
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| Overall design |
GAS6-induced MerTK+ macrophages of WT or ATF3-CKO mice were harvested 24 hours following HR and performed to further analysis.
For microarray profiling, the BMDMs total RNA was harvested using TRIzol reagent (Invitrogen, Carlsbad, Canada) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using UV-Vis Spectrophotometer (Thermo, NanoDrop 2000, USA) at the absorbance of 260 nm. The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix GeneChip, Santa Clara CA, USA), which contains 65957 gene-level probe sets.
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| Contributor(s) |
Li Y, Shao Y |
| Citation(s) |
39195894 |
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| Submission date |
Oct 26, 2023 |
| Last update date |
Sep 30, 2024 |
| Contact name |
李 玉琳 |
| Organization name |
Beijing Institute of Heart Lung and Blood Vessel Diseases
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| Street address |
2 Anzhen Rd, Chaoyang District,
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| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
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| Platforms (1) |
| GPL20266 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1032575 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE246338_RAW.tar |
137.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
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