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| Status |
Public on Oct 10, 2015 |
| Title |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus ATCC 14579 |
| Organism |
Bacillus cereus ATCC 14579 |
| Experiment type |
Expression profiling by array
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| Summary |
Planktonic and biofilm cells of Bacillus cereus ATCC 14579 and ATCC 10987 were studied using microscopy and transcriptome analysis. By microscopy, clear differences could be observed between biofilm and planktonic cells as well as between the two strains. By using hierarchical clustering of the transcriptome data, little difference was observed between the biofilm cells of B. cereus ATCC 14579 and ATCC 10987. Different responses between biofilm and planktonic cells could be identified using transcriptome analysis. Biofilm formation seemed to cause a shift in metabolism with up- or down-regulation of genes involved in different metabolic pathways. Genes involved in motility were down-regulated. No clear up-regulation related to capsular or extracellular polysaccharides was observed. Sporulation was observed in biofilm cells using microscopy, which was corroborated with up-regulation of genes involved in sporulation in biofilm cells. The results obtained in this study provide insight in general and strain specific behavior of B. cereus cells in multicellular communities.
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| Overall design |
Planktonic cell samples were isolated at mid-logarithmic growth and stationary growth phase. Biofilm cell samples were isolated after 12 and 24 hours of growth. Each conditions was compared to the three other conditions.The experiments were performed in duplicate and the duplicate samples were hybridized with a dye-swap.
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| Contributor(s) |
Wijman J, Mols M, Tempelaars M, Abee T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Oct 11, 2010 |
| Last update date |
Oct 10, 2015 |
| Contact name |
Janneke Wijman |
| Organization name |
Wageningen UR
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| Lab |
Food Microbiology
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| Street address |
Bomenweg 2
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| City |
Wageningen |
| ZIP/Postal code |
6701 HD |
| Country |
Netherlands |
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| Platforms (1) |
| GPL7680 |
Bacillus cereus Bc14579 - 2nd design (2x11K) |
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| Samples (12)
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| GSM606627 |
Biofilm 12 h compared to biofilm 24 h |
| GSM607048 |
24 hour biofilm compared to 12hour biofilm |
| GSM607049 |
12 hour biofilm compared to stationary phase cells |
| GSM607050 |
stationary phase cells compared to 12 h biofilm |
| GSM607051 |
12 hour biofilm compared to mid-log cells |
| GSM607052 |
Mid-log cells compared to 24 hour biofilm |
| GSM607053 |
mid-log cells compared to stationary cells |
| GSM607054 |
Stationary phase cells compared to mid-logarithmic phase cells |
| GSM607055 |
24 hour biofilm compared to stationary phase cells |
| GSM607056 |
Stationary phase cells compared to 24 hour biofilm cells |
| GSM607057 |
12 hour biofilm cells compared to mid-log cells |
| GSM607063 |
Mid-log cells compared to 12 hour biofilm cells |
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| This SubSeries is part of SuperSeries: |
| GSE24635 |
Comparative transcriptome analysis of biofilm and planktonic cells of Bacillus cereus |
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| Relations |
| BioProject |
PRJNA133577 |
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