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| Status |
Public on Feb 09, 2024 |
| Title |
Single cell transcriptome analysis of the THY-Tau22 mouse model of Alzheimer's disease reveals sex-dependent dysregulations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Alzheimer's disease (AD) is characterized by the accumulation of amyloid-beta and tau proteins in vulnerable brain regions. While the main pathological hallmarks of AD are shared across the sexes, a diverse array of sex differences have been documented for both AD-associated symptoms and molecular characteristics. To gain insights into AD-associated molecular changes and their sex-dependence for tau pathology in the cortex as one of the most severely affected brain regions, we performed single-cell gene activity profiling for the THY-Tau22 mouse model. By studying cell-type specific and cell-type agnostic AD-related changes and their sex-dimorphism for single genes, pathways and cellular sub-networks, we aimed to identify both statistically significant alterations and interpret the upstream mechanisms that control them.
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| Overall design |
The study used the THY-Tau22 transgenic mouse model of Alzheimer's disease, which overexpresses a mutant human tau protein. Single-cell RNA-seq was performed on brain cortex samples from 7-month old THY-Tau22 mice and wildtype control mice (5 mice per genotype and sex). The experimental conditions compared were: THY-Tau22 transgenic males (n=5), Wildtype control males (n=5), THY-Tau22 transgenic females (n=5), and Wildtype control females (n=5).
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| Web link |
https://doi.org/10.1038/s41420-024-01885-9
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| Contributor(s) |
Ali M, Garcia P, Lunkes LP, Sciortino A, Thomas M, Heurtaux T, Grzyb K, Halder R, Coowar D, Skupin A, Buée L, Blum D, Buttini M, Glaab E |
| Citation(s) |
38453894 |
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| Submission date |
Oct 10, 2023 |
| Last update date |
Mar 08, 2024 |
| Contact name |
Enrico Glaab |
| E-mail(s) |
enrico.glaab@uni.lu
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| Organization name |
University of Luxembourg
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| Street address |
6, avenue du Swing
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| City |
Belvaux |
| State/province |
Capellen |
| ZIP/Postal code |
L-4367 |
| Country |
Luxembourg |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA1026649 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE245035_RAW.tar |
42.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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