|
| Status |
Public on Jun 19, 2024 |
| Title |
RNA Sequencing of murine MPNST Tumors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We utilized three murine MPNST cell lines. 67C-4 and #5NPCIS cell lines are derived from spontaneously arising tumors in mice mutated for NF1 and p53 (so-called NP-cis mice; PMID: 12154062). SN4-4 cells originated from MPNST tumors induced by injecting adenovirus containing Cas9 and guide RNA for Nf1 and p53 into the sciatic nerves, resulting in NF1/p53-null MPNST (PMID: 32456131). We implanted 5 x 10^6 murine MPNST cells, subcutaneously, into the flanks of 6-8 weeks old gender-matched C57BL/6 mice (for 67C-4 and #5NPCIS) and BALB/c mice (for SN4-4 mice) to generate MPNST tumors. All mice were procured from Envigo. Upon reaching a size of 300-500 mm3, the mice were euthanized to harvest the tumors, which were subsequently flash frozen using liquid nitrogen and processed for RNA sequencing.
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| |
|
| Overall design |
Gene Expression Analysis of murine Malignant Peripheral Nerve Sheath Tumors (MPNST)
|
| Web link |
https://pubmed.ncbi.nlm.nih.gov/39044819/
|
| |
|
| Contributor(s) |
Paudel SN, Miller K, Garfinkle E |
| Citation(s) |
39044819 |
| |
| Submission date |
Oct 06, 2023 |
| Last update date |
Sep 18, 2024 |
| Contact name |
Katherine E Miller |
| E-mail(s) |
katherine.miller@nationwidechildrens.org
|
| Organization name |
Nationwide Children's Hospital
|
| Department |
Institute for Genomic Medicine
|
| Street address |
700 Children's Dr.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (11)
|
|
| Relations |
| BioProject |
PRJNA1024972 |
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