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| Status |
Public on Nov 23, 2011 |
| Title |
Murine bone marrow-derived mast cells (BMMCs) transduced with pAPM lentiviruses overexpressing miR-221 vs control overexpressing miR-221m. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Bone marrow-derived mast cells were derived from three different female C57Bl/6 mice of similar age. Cells were transduced with pAPM lentiviruses either overexpressing microRNA-221 or a non functional microRNA-221 mutant, termed miR-221m, where four point-mutations were introduced in the seed sequence of miR-221. The samples overexpressing miR-221m were used as references for the corresponding miR-221 overexpressing BMMCs from the same mouse (mouse matched reference). The experiment was performed in order to gain more insights about the molecular mechanisms underlying miR-221 effects in mast cells.
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| Overall design |
Two-condition experiment, BMMC overexpressing miR-221 vs BMMC overexpressing miR-221m. Biological replicates: 3 replicates overexpressing miR-221, 3 control replicates overexpressing miR-221m.
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| Contributor(s) |
Monticelli S, Mayoral RJ |
| Citation(s) |
22022537 |
| |
| Submission date |
Sep 30, 2010 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Silvia Monticelli |
| E-mail(s) |
silvia.monticelli@irb.unisi.ch
|
| URL |
http://www.irb.ch
|
| Organization name |
Institute for Research in Biomedicine
|
| Lab |
Molecular Immunology
|
| Street address |
Via Vincenzo Vela, 6
|
| City |
Bellinzona |
| ZIP/Postal code |
6500 |
| Country |
Switzerland |
| |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
|
| Samples (3) |
| GSM602668 |
BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 1 |
| GSM602669 |
BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 2 |
| GSM602670 |
BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 3 |
|
| Relations |
| BioProject |
PRJNA132755 |
