GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE24462 Query DataSets for GSE24462
Status Public on Nov 23, 2011
Title Murine bone marrow-derived mast cells (BMMCs) transduced with pAPM lentiviruses overexpressing miR-221 vs control overexpressing miR-221m.
Organism Mus musculus
Experiment type Expression profiling by array
Summary Bone marrow-derived mast cells were derived from three different female C57Bl/6 mice of similar age. Cells were transduced with pAPM lentiviruses either overexpressing microRNA-221 or a non functional microRNA-221 mutant, termed miR-221m, where four point-mutations were introduced in the seed sequence of miR-221. The samples overexpressing miR-221m were used as references for the corresponding miR-221 overexpressing BMMCs from the same mouse (mouse matched reference). The experiment was performed in order to gain more insights about the molecular mechanisms underlying miR-221 effects in mast cells.
Overall design Two-condition experiment, BMMC overexpressing miR-221 vs BMMC overexpressing miR-221m. Biological replicates: 3 replicates overexpressing miR-221, 3 control replicates overexpressing miR-221m.
Contributor(s) Monticelli S, Mayoral RJ
Citation(s) 22022537
Submission date Sep 30, 2010
Last update date Jan 12, 2017
Contact name Silvia Monticelli
Organization name Institute for Research in Biomedicine
Lab Molecular Immunology
Street address Via Vincenzo Vela, 6
City Bellinzona
ZIP/Postal code 6500
Country Switzerland
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (3)
GSM602668 BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 1
GSM602669 BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 2
GSM602670 BMMC-pAPM-miR-221 vs BMMC-pAPM-miR-221m replicate 3
BioProject PRJNA132755

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE24462_RAW.tar 10.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap