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| Status |
Public on Dec 20, 2023 |
| Title |
Gene expression profile of murine thymic B cells in the presence or absence of CD40 signaling |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The thymus contains some B cells that are located at the corticomedullary junction. We found that these thymic B cells express and present the water channel protein AQP4 and are necessary and sufficient to mediate the negative selection of AQP4-reactive thymocytes. In order to express and present AQP4, thymic B cells need to be licensed through a CD40 signal. Here, we tested the significance of CD40 signaling in thymic B cells for the expression and presentation of distinct autoantigens (besides AQP4) to orchestrate thymocyte selection for these autoantigens.
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| Overall design |
2000 B cells per sample were sorted from the thymus of naive, untreated wild type (WT, C57BL/6, Charles River) or CD40KO (JAX strain 002927) mice aged 6-8 weeks (5 mice per genotype) and then subjected to bulk RNAseq. After the initial run, the same libraries were sequenced again to increase overall sequencing depth.
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| Contributor(s) |
Korn T, Afzali A, Nirschl L, Öllinger R, Muschaweckh A, Bonafonte Pardàs I, Sie C |
| Citation(s) |
38383779 |
| |
| Submission date |
Sep 29, 2023 |
| Last update date |
Feb 22, 2024 |
| Contact name |
Thomas Korn |
| E-mail(s) |
thomas.korn@tum.de, c.sie@tum.de
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| Organization name |
Technical University of Munich
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| Department |
Experimental Neuroimmunology
|
| Street address |
Ismaninger Str. 22
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| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (10)
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| GSM7814261 |
WT B cells (IgM+, IgD-) Biological Replicate 1 |
| GSM7814262 |
WT B cells (IgM+, IgD-) Biological Replicate 2 |
| GSM7814263 |
WT B cells (IgM+, IgD-) Biological Replicate 3 |
| GSM7814264 |
WT B cells (IgM+, IgD-) Biological Replicate 4 |
| GSM7814265 |
WT B cells (IgM+, IgD-) Biological Replicate 5 |
| GSM7814266 |
CD40KO B cells (Total) Biological Replicate 1 |
| GSM7814267 |
CD40KO B cells (Total) Biological Replicate 2 |
| GSM7814268 |
CD40KO B cells (Total) Biological Replicate 3 |
| GSM7814269 |
CD40KO B cells (Total) Biological Replicate 4 |
| GSM7814270 |
CD40KO B cells (Total) Biological Replicate 5 |
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| Relations |
| BioProject |
PRJNA1022474 |
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