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| Status |
Public on Dec 22, 2023 |
| Title |
Parallel neural pathways control sodium consumption and taste valence |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed single-cell RNA-seq (10x Chromium 3' v.3.1) on dissociated single cell suspensions from mouse (C57BL/6) osmosensory brain nuclei - subfornical organ (SFO) to determine transcriptomic cell types and neuronal activation patterns. By performing single cell RNA-seq based stimulus to cell-type mapping, we captured the endogenous immediate early gene expression triggered by distinct physiological state stimuli in SFO cells. We identified a unique excitatory neuron type in SFO that is uniquely tuned to sodium deficiency.
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| Overall design |
For stimulus to cell-type mapping experiments we used 15-20 animals per condition. Both male and female animals were used in equal measure. All animals were 7-8 weeks of age. Prior to tissue extraction, animals were subjected to one of the following experimental treatments: a) wated satiation: ad libitum access to food and water); b) sodium depletion: intraperitoneal (i.p.) injection of furosemide at a dose of 50 mg/kg bodyweight followed by one day maintainance on a low-sodium diet.
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| Web link |
https://www.sciencedirect.com/science/article/pii/S0092867423011716#sec4
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| Contributor(s) |
Zhang Y, Wang T, Pool A, Oka Y |
| Citation(s) |
37989313 |
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| Submission date |
Sep 28, 2023 |
| Last update date |
Dec 23, 2023 |
| Contact name |
Tongtong Wang |
| E-mail(s) |
tongtong@caltech.edu
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| Organization name |
California Institute of Technology
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| Street address |
1200 E California Blvd
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| City |
Pasadena |
| State/province |
CA |
| ZIP/Postal code |
91125 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA1021992 |
