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| Status |
Public on Jan 20, 2011 |
| Title |
Systematic meta-analysis and replication of genome-wide expression studies identifies molecular pathways of Parkinson’s disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of human dopamine (DA) from postmortem brains of 8 patients with Parkinson’s disease (PD). Results provide insight into the molecular processes perturbed in the PD substantia nigra.
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| Overall design |
Human dopamine (DA) neurons from 8 PD and 9 control subjects were obtained. Double-stranded complementary DNA was made with a biotinylated T7(dT)-24 primer. Biotinylated complementary RNA was fragmented and hybridized to Affymetrix human genome U133_X3P microarrays. The Affymetrix .CEL files were normalized to “all probe sets” in a standardized matter, and scaled to 100 by the MAS5 algorithm implemented in the Bioconductor package.
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| |
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| Contributor(s) |
Cantuti-Castelvetri I, Scherzer CR, Zheng B |
| Citation(s) |
20926834 |
| |
| Submission date |
Sep 27, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Bin Zheng |
| Organization name |
Brigham and Women's hospital
|
| Department |
Center for Neurologic Diseases
|
| Street address |
65 Landsdowne Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1352 |
[U133_X3P] Affymetrix Human X3P Array |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA132861 |
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