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| Status |
Public on Aug 31, 2011 |
| Title |
Secreted Factors from Staphylococcus aureus Biofilm and Planktonic Cultures Differentially Impact Human Keratinocytes in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Interactions between human keratinocytes and secreted factors from Staphylococcus aureus biofilm were investigated using microarray analysis. Relative to control cells, biofilm-secreted factors upregulated cytokine and chemokine genes in keratinocytes. Genes associated with DNA damage and oxidative stress were also induced in keratinocytes treated with secreted factors from S. aureus. Here we show that secreted factors from S. aureus biofilm cultures differentially impact several aspects of wound healing processes.
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| Overall design |
Human keratinocytes were grown in co-culture with mature S. aureus biofilms for 24 hours. Keratinocytes exposed to S. aureus biofilm were analyzed in quadruplicate. Control cells were also analyzed in quadruplicate. Dye-swaps were performed.
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| Contributor(s) |
Secor PR, James GA, Fleckman P, Olerud JE, McInnerney K, Dowd SE, Stewart PS |
| Citation(s) |
21693040 |
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| Submission date |
Sep 25, 2010 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Kate McInnerney |
| E-mail(s) |
kmcinnerney@montana.edu
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| Phone |
406-994-5666
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| Fax |
406-994-4926
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| URL |
http://cores.montana.edu
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| Organization name |
Montana State University
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| Department |
Microbiology
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| Lab |
Functional Genomics Facility
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| Street address |
109 Lewis Hall
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| City |
Bozeman |
| State/province |
MT |
| ZIP/Postal code |
59717 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (14)
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| GSM604536 |
Treated, rep1, dye-swap |
| GSM604537 |
Treated, rep2 |
| GSM604538 |
Control, rep2 |
| GSM604539 |
Treated, rep3 |
| GSM604540 |
Control, rep3 |
| GSM604541 |
Control, rep3, dye-swap |
| GSM604542 |
Treated, rep3, dye-swap |
| GSM604543 |
Treated, rep4 |
| GSM604544 |
Control, rep4 |
| GSM604545 |
Control, rep4, dye-swap |
| GSM604546 |
Treated, rep4, dye-swap |
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| Relations |
| BioProject |
PRJNA132965 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE24370_RAW.tar |
95.1 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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