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| Status |
Public on Aug 21, 2024 |
| Title |
Fungal Dysbiosis in Post-Influenza MRSA Infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A leading cause of morbidity and mortality during influenza infection is the development of a secondary bacterial pneumonia, which is appropriately treated with antibiotics. In the absence of a bacterial superinfection, prescribing antibiotics is not indicated but has nevertheless become a common clinical practice for those presenting with a respiratory viral illness. We found that antibiotic use during an antecedent influenza infection impaired the lung innate immunologic defenses toward a secondary challenge with methicillin-resistantStaphylococcus aureus(MRSA). The antibiotics perturbed the gut microbiome causing a fungal dysbiosis that drives an increase in lung eosinophils. We also demonstrate eosinophils, through the release of major basic protein, impair macrophage ability to clear MRSA. Moreover, we provide clinical evidence that eosinophils positively correlate with antibiotic use and worsened outcomes in patients hospitalized for viral infections. Altogether, our work establishes a counterproductive effect of antibiotic treatment during influenza infection that have negative immunologic consequences in the lungs thereby increasing the risk of developing a secondary bacterial infection.
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| Overall design |
Single cells were FACS sorted to gate out dead cells and subject for single cell RNAseq experiments, 4 mouse lungs for each group. Each repilicate was barcoded by Totalseq-A HTO antibody.
Please note that the .h5 data file linked to each sample is also associated with the corresponding HTO sample.
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| Contributor(s) |
Chen P, Parimon T, Yao C, Bora S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 19, 2023 |
| Last update date |
Aug 22, 2024 |
| Contact name |
Changfu Yao |
| E-mail(s) |
changfu.yao@cshs.org
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| Phone |
424-315-4333
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| Organization name |
cedars-sinai medical center
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| Department |
Lung and Regenerative Medicine Institutes
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| Lab |
Stripp Lab
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| Street address |
8700 Beverly Blvd. AHSP Room A9401
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| City |
los angeles |
| State/province |
Ca |
| ZIP/Postal code |
90048 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA1018896 |
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