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| Status |
Public on Jul 01, 2024 |
| Title |
METTL7B is an essential epigenetic regulator of lineage specification in glioblastoma [DNAme] |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
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| Summary |
Glioblastomas are the most common malignant brain tumours in adults; they are highly aggressive, heterogeneous and plastic. We have identified METTL7B as an essential regulator of lineage specification in glioblastoma, with impact on both tumour size and invasiveness in in vivo models. Single cell transcriptomic analysis of these tumours and of cerebral organoids derived from expanded potential stem cells overexpressing METTL7B identified a regulatory role for the gene in the neural stem cells to astrocyte differentiation trajectory. Mechanistically, METTL7B downregulates the expression of key neuronal differentiation players, including SALL2, via DNA methylation and post-translational modifications of histone marks.
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| Overall design |
DNA methylation analysis of GIC19 and iNSC19 upon silencing or overexpression of METTL7B respectively (n = 4 replicates per group).
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| Contributor(s) |
Marino S, Constantinou M, Nicholson J, Maniati E, Badodi S |
| Citation(s) |
38848215 |
| |
| Submission date |
Sep 13, 2023 |
| Last update date |
Jul 02, 2024 |
| Contact name |
James Nicholson |
| E-mail(s) |
james.nicholson@qmul.ac.uk
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| Organization name |
Queen Mary University of London
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| Department |
Blizard Institute
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| Street address |
4 Newark St
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| City |
London |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE243132 |
METTL7B is an essential epigenetic regulator of lineage specification in glioblastoma |
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| Relations |
| BioProject |
PRJNA1016410 |
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