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| Status |
Public on Jan 28, 2024 |
| Title |
Identification of PRMT1 as a suppressor of MHC-I and anti-tumour immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To understand the mechanisms by which Prmt1 knockout or inhibition sensitizes B16F10 to T cell mediated killing.
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| Overall design |
B16F10 cells were pre-treated with Prmt1-inhibitor GSK3368715 (2.5 µM) for 2 days. Afterwards, untreated wildtype (controls), Prmt1-inhibitor treated or Prmt1-/- B16F10 were cultured for one more day in the presence or absence of mouse IFNγ (1 ng/ml). There are three independent replicates for each condition. Total RNA was then extracted, quantitated with 4200 Tapestation and finally, 1 µg of total RNA per sample was subjected to NGS library preparation (TruSeq RNA). NGS libraries were pooled and sequenced using a P2 100-cycle Illumina sequencing kit on the NextSeq 2000 sequencing system with paired-end 66bp reads.
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| |
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| Contributor(s) |
Djajawi TM, Neil L, Pal B, Liao Y, Shi W, Kearney CJ |
| Citation(s) |
38401121 |
| |
| Submission date |
Sep 12, 2023 |
| Last update date |
Apr 28, 2024 |
| Contact name |
Wei Shi |
| E-mail(s) |
Wei.Shi@onjcri.org.au
|
| Organization name |
Olivia Newton John Cancer Research Institute
|
| Department |
Bioinformatics and Cancer Genomics
|
| Street address |
Level 5, ONJ Cancer Centre, 145 Studley Rd
|
| City |
Heidelberg |
| State/province |
VIC |
| ZIP/Postal code |
3084 |
| Country |
Australia |
| |
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| Platforms (1) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA1016038 |
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