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| Status |
Public on Sep 22, 2023 |
| Title |
Transcriptional regulation of human dorsal root ganglia cultures by type 2 cytokines |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cutaneous afferent neurons express receptors for type 2 cytokines, including interleukins (IL)-4, IL-13, and IL-33. These neuronal cytokine receptors were found to be required in several murine models of itch. The objective of the present study was to determine if type 2 cytokine stimulation sensitizes sensory neurons to future itch stimuli in a fully human ex vivo system. Human dorsal root ganglia were isolated and dissociated to establish ex vivo cultures and subjected to 24 hours of type 2 cytokine exposure with either vehicle, IL-4, IL-13, IL-33, or IL-4 and IL-13 together. After 24 hours, cells were harvested and RNA extracted and sequenced to determine the transcriptomic effects of type 2 cytokine exposure.
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| Overall design |
Cultures were stimulated with 500 nM IL-4, IL-13, IL-33 or the combination of IL-4 and IL-13 for 24 hours. Vehicle alone and a cocktail of inflammatory mediators "CIS" were as negative and positive controls.
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| Web link |
http://doi: 10.3389/fnmol.2023.1258823
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| Contributor(s) |
Mack MR |
| Citation(s) |
37868811 |
| BioProject |
PRJNA1008658 |
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| Submission date |
Sep 06, 2023 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Madison R Mack |
| Organization name |
Sanofi
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| Department |
Immunology & Inflammation Research
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| Street address |
350 Water St
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| City |
Cambridge |
| State/province |
Massachusetts |
| ZIP/Postal code |
02141 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (16)
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