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| Status |
Public on Sep 12, 2023 |
| Title |
Potential role of Bcl2 in lipid metabolism and synaptic dysfunction of age-related hearing loss |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Age-related hearing loss (ARHL) is a prevalent disease that affects millions of people worldwide. BCL-2 gene plays a tremendous role in cellular senescence, and it was confirmed that the BCL-2 family is closely associated with neurological disorders and ARHL. In addition to modulating apoptosis and oxidative stress, as well as other effects, this study we report here that BCL-2 plays a crucial position in regulating phospholipid and lipid metabolism in the developing cochlear tissues of rats and a sensory epithelial cell line(OC1), including the cholesterol synthesis pathway that is differentially expressed by genes such as LSS,NSDHL, and CYP51, which further demonstrates the mechanism of the action of BCL-2 in hair cells. In conclusion our data provide a new molecular framework for the regulatory mechanism of BCL-2 down-regulation in senescent cells. These findings provide valuable insights into the molecular mechanisms of ARHL and may set the stage for new therapeutic approaches for this prevalent age-related disease.
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| Overall design |
To investigate the potential mechanism of BCL-2 action in ARHL, we established OC1 cells with knocked down BCL-2 targeting sequences by siRNA. We performed gene expression profiling using RNA-seq data obtained from cells that knocked down the target gene in different targeting sequences. A comparison of the gene expression profiling analysis of RNA-seq data from OC1 cells was performed.
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| Contributor(s) |
Fan C, Liu Y, Zhang H |
| Citation(s) |
37813166 |
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| Submission date |
Sep 05, 2023 |
| Last update date |
Dec 21, 2023 |
| Contact name |
Yue Liu |
| E-mail(s) |
A1785206@126.com
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| Organization name |
Longgang E.N.T Hospital & Shenzhen Key Laboratory of E.N.T, Institute of E.N.T
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| Street address |
No. 3004, Longgang Section, Longgang Avenue, Longgang District
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| City |
Shenzhen |
| ZIP/Postal code |
518172 |
| Country |
China |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1013359 |
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