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| Status |
Public on Sep 11, 2024 |
| Title |
Acyl-coA Binding Protein neutralization improves cancer immunosurveillance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The plasma concentrations of acyl coenzyme A binding protein (ACBP, also known as diazepam-binding inhibitor, DBI, or ‘endozepine’) increase with age and obesity, two parameters that are also the most important risk factors for cancer. In mice bearing MCA205 fibrosarcoma, antibody mediated ACBP/DBI neutralization enhanced the anticancer T-cell response in the context of chemoimmunotherapy. T-cells infiltrating MCA205 tumors were sorted and submitting to single-cell TCR sequencing and single-cell RNA sequencing (scRNAseq) to identify the mechanisms driving this improvement.
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| Overall design |
C57Bl/6 mice were injected with 3E5 MCA205 cells subcutaneously. 8 days after injection they were treated intraperitoneally with one cycle of chemotherapy (10 mg/kg oxaliplatin) followed, 8 days later, by immunotherapy (200 µg anti-PD1). Simultaneously, half the mice were treated with anti-ACBP/DBI neutralizing antibody (n = 3), the other half with isotype control (n=3) (2.5 mg/kg every other day, from two days before chemotherapy to the day prior to immunotherapy). Tumors were collected two days after immunotherapy, dissociated and fluorescently labelled to sort CD4+ and CD8+ T cells. A CD4:CD8 ratio of 1:1 was analyzed by TCR/scRNAseq.
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| Contributor(s) |
Montégut L, Mena Osuna R, Martins I, Piaggio E, Kroemer G |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 31, 2023 |
| Last update date |
Sep 12, 2024 |
| Contact name |
Noémie Robil |
| E-mail(s) |
noemie.robil@genosplice.com
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| Organization name |
GenoSplice technology
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| Department |
Paris Biotech Santé
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| Street address |
29, rue du Faubourg Saint-Jacques
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| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1011354 |
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