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| Status |
Public on May 14, 2024 |
| Title |
The ATHB2 microProtein affects plant development and the shade avoidance response (PEAT) |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Other
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| Summary |
Plants display remarkable adaptability to varying light conditions, crucial for their optimal growth and development. Here, we investigate the functional significance of ATHB2-miP, a microProtein arising from an alternative transcript of the ATHB2 gene, in both white light and shade environments, supported by comprehensive phenotyping experiments. ATHB2, a homeodomain-leucine zipper transcription factor, is known for its involvement in growth regulation. By employing genetic, transcriptomic analyses, and phenotyping experiments, we dissect the regulatory role of ATHB2-miP. Transcriptomics unravel genes differentially induced and repressed upon ATHB2-miP expression, elucidating its impact on growth-related pathways. Notably, ATHB2-miP's expression responds to shade conditions, suggesting its role in light-mediated responses. Our research underscores ATHB2-miP's multifaceted contribution to plant growth and development, emphasizing its potential as a pivotal player in light-responsive gene regulation.
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| Overall design |
We conducted transcriptomics, phenotyping studies, genetic analyses and protein interaction experiments on mutant lines generated by CRISPR-Cas9 where the microProein was deleted, a t-knockout line of the full ATHB2 gene and a line overexpressing the microProtein under the control of a 35S promoter.
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| Contributor(s) |
Wenkel S, Edwards AK, Chiurazzi MJ |
| Citation missing |
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| Submission date |
Aug 30, 2023 |
| Last update date |
May 21, 2024 |
| Contact name |
Stephan Wenkel |
| E-mail(s) |
stephan.wenkel@umu.se
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| Organization name |
Umeå Plant Science Center
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| Street address |
Parallellvägen 21
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| City |
Umeå |
| ZIP/Postal code |
907 36 |
| Country |
Sweden |
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| Platforms (1) |
| GPL17639 |
Illumina HiSeq 2500 (Arabidopsis thaliana) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE241976 |
The ATHB2 microProtein affects plant development and the shade avoidance response |
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| Relations |
| BioProject |
PRJNA1010837 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE241975_resultPEAT_interesting_selected_fisherextract.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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