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Series GSE241974 Query DataSets for GSE241974
Status Public on May 14, 2024
Title The ATHB2 microProtein affects plant development and the shade avoidance response (RNA-Seq)
Organism Arabidopsis thaliana
Experiment type Expression profiling by high throughput sequencing
Summary Plants display remarkable adaptability to varying light conditions, crucial for their optimal growth and development. Here, we investigate the functional significance of ATHB2-miP, a microProtein arising from an alternative transcript of the ATHB2 gene, in both white light and shade environments, supported by comprehensive phenotyping experiments. ATHB2, a homeodomain-leucine zipper transcription factor, is known for its involvement in growth regulation. By employing genetic, transcriptomic analyses, and phenotyping experiments, we dissect the regulatory role of ATHB2-miP. Transcriptomics unravel genes differentially induced and repressed upon ATHB2-miP expression, elucidating its impact on growth-related pathways. Notably, ATHB2-miP's expression responds to shade conditions, suggesting its role in light-mediated responses. Our research underscores ATHB2-miP's multifaceted contribution to plant growth and development, emphasizing its potential as a pivotal player in light-responsive gene regulation.
 
Overall design We conducted transcriptomics, phenotyping studies, genetic analyses and protein interaction experiments on mutant lines generated by CRISPR-Cas9 where the microProein was deleted, a t-knockout line of the full ATHB2 gene and a line overexpressing the microProtein under the control of a 35S promoter.
 
Contributor(s) Wenkel S, Edwards AK, Chiurazzi MJ
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Submission date Aug 30, 2023
Last update date May 21, 2024
Contact name Stephan Wenkel
E-mail(s) stephan.wenkel@umu.se
Organization name Umeå Plant Science Center
Street address Parallellvägen 21
City Umeå
ZIP/Postal code 907 36
Country Sweden
 
Platforms (1)
GPL21785 Illumina HiSeq 4000 (Arabidopsis thaliana)
Samples (27)
GSM7746959 WT_WL_rep1
GSM7746960 WT_WL_rep2
GSM7746961 WT_WL_rep3
This SubSeries is part of SuperSeries:
GSE241976 The ATHB2 microProtein affects plant development and the shade avoidance response
Relations
BioProject PRJNA1010838

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241974_OX_0_counts_merged.txt.gz 240.7 Kb (ftp)(http) TXT
GSE241974_OX_45_counts_merged.txt.gz 242.4 Kb (ftp)(http) TXT
GSE241974_OX_90_counts_merged.txt.gz 239.8 Kb (ftp)(http) TXT
GSE241974_WT_0_counts_merged.txt.gz 235.7 Kb (ftp)(http) TXT
GSE241974_WT_45_counts_merged.txt.gz 237.2 Kb (ftp)(http) TXT
GSE241974_WT_90_counts_merged.txt.gz 239.4 Kb (ftp)(http) TXT
GSE241974_tHAT4_0_counts_merged.txt.gz 236.3 Kb (ftp)(http) TXT
GSE241974_tHAT4_45_counts_merged.txt.gz 236.4 Kb (ftp)(http) TXT
GSE241974_tHAT4_90_counts_merged.txt.gz 239.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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