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Series GSE241756 Query DataSets for GSE241756
Status Public on Dec 10, 2023
Title Plasma miRNAs as biomarkers of epilepsy
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Epilepsy frequently develops as a result of brain insult, for example brain injury or stroke. Currently there are no tools allowing us to predict which trauma patients will eventually develop epilepsy. There is evidence that microRNAs levels are altered in plasma, making them attractive candidates for peripheral biomarkers of epilepsy.
We analyzed miRNA levels in plasma samples using Affymetrix microarrays 4.1 and performed comparative analysis of samples. We performed 3 comparisons: i) control animals vs. Status epilepticus animals, ii) animals which developed first spontaneous seizure around 7 days post stimulation (EARLY), or later after 21days post stimulation (LATE), iii) animals that had developed seizures at given timepoint (EPI) and animals that did not experience seizures by given timepoint (NONEPI).
 
Overall design We draw the blood from rats after electrical stimulation of amygdala, which induces status epilepticus, at 4 timepoints: 14, 30, 60 and 90 days after status epilepticus. Later RNA was isolated from all the samples at the same time and microarray 4.1 were used in agreement with the producer's protocol, to test levels of each miRNA.
 
Contributor(s) Szydlowska K, Lukasiuk K
Citation(s) 38184716
Submission date Aug 28, 2023
Last update date Jan 17, 2024
Contact name Kinga Szydlowska
E-mail(s) k.szydlowska@nencki.edu.pl
Organization name Nencki Institute of Experimental Biology
Lab Epileptogenesis Lab
Street address Pasteura 3
City Warsaw
ZIP/Postal code 02-093
Country Poland
 
Platforms (1)
GPL30571 [miRNA-4] Affymetrix Rat miRNA-4 Array [Brain Array V21]
Samples (92)
GSM7734783 plasma sample from control rat at 14 days post status epilepticus, biological replicate 1 [14D_CTRL_1]
GSM7734784 plasma sample from control rat at 14 days post status epilepticus, biological replicate 2 [14D_CTRL_2]
GSM7734785 plasma sample from control rat at 14 days post status epilepticus, biological replicate 3 [14D_CTRL_3]
Relations
BioProject PRJNA1009812

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE241756_RAW.tar 69.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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